Cryo-EM

SciLifeLab National facility since 2016.

61 publications

Year 2020

Distinct pre-initiation steps in human mitochondrial translation
Khawaja A, Itoh Y, Remes C, ..., Amunts A, Rorbach J
Nat Commun 11 (1) 1-10 [2020-12-00; online 2020-06-10]
Cryo-EM [Service]
Cryo‐EM structure of native human uromodulin, a zona pellucida module polymer
Stsiapanava A, Xu C, Brunati M, ..., Wu B, Jovine L
EMBO J - (-) e106807 [2020-11-16; online 2020-11-16]
Cryo-EM [Collaborative]
Structure and elevator mechanism of the mammalian sodium/proton exchanger NHE9
Winkelmann I, Matsuoka R, Meier PF, ..., Beckstein O, Drew D
EMBO J - (-) - [2020-10-29; online 2020-10-29]
Cryo-EM [Service]
Type III ATP synthase is a symmetry-deviated dimer that induces membrane curvature through tetramerization.
Flygaard RK, Mühleip A, Tobiasson V, Amunts A
pii: 10.1038/s41467-020-18993-6
Nat Commun 11 (1) 5342 [2020-10-22; online 2020-10-22]
Cryo-EM [Service]
Structural basis of mitochondrial translation
Aibara S, Singh V, Modelska A, Amunts A
Elife 9 (-) 1-17 [2020-08-19; online 2020-08-19]
Cryo-EM [Service]
Structure of a minimal photosystem I from the green alga Dunaliella salina
Perez-Boerema A, Klaiman D, Caspy I, ..., Amunts A, Nelson N
NPLANTS 6 (3) 321-327 [2020-03-02; online 2020-03-02]
Cryo-EM [Service]
Capsid Structure of a Marine Algal Virus of the Order Picornavirales
Munke A, Kimura K, Tomaru Y, Okamoto K
J Virol 94 (9) - [2020-02-05; online 2020-04-16]
Cryo-EM [Service]

Year 2019

Structure-based mechanism for activation of the AAA+ GTPase McrB by the endonuclease McrC
Nirwan N, Itoh Y, Singh P, ..., Amunts A, Saikrishnan K
Nat Commun 10 (1) 1-9 [2019-12-00; online 2019-07-11]
Cryo-EM [Service]
Apolipoprotein E impairs amyloid‐β fibril elongation and maturation
Islam T, Gharibyan AL, Golchin SA, ..., Olofsson L, Olofsson A
FEBS J - (-) - [2019-10-09; online 2019-10-09]
Cryo-EM [Service]
Cryo-EM structure of the activated RET signaling complex reveals the importance of its cysteine-rich domain
Bigalke JM, Aibara S, Roth R, ..., Amunts A, Sandmark J
Sci Adv 5 (7) eaau4202 [2019-07-00; online 2019-07-31]
Cryo-EM [Service]
Toxic Activation of an AAA+ Protease by the Antibacterial Drug Cyclomarin A
Maurer M, Linder D, Franke KB, ..., Bukau B, Mogk A
Cell Chem Biol - (-) - [2019-06-00; online 2019-06-00]
Cryo-EM [Service]
Assembly mechanisms of the bacterial cytoskeletal protein FilP
Javadi A, Söderholm N, Olofsson A, Flärdh K, Sandblad L
Life Sci. Alliance 2 (3) e201800290 [2019-06-00; online 2019-06-26]
Cryo-EM [Collaborative]
Zinc depletion does not necessarily induce ribosome hibernation in mycobacteria
Tobiasson V, Dow A, Prisic S, Amunts A
Proc Natl Acad Sci USA 116 (7) 2395-2397 [2019-02-12; online 2019-01-25]
Cryo-EM [Service]

Year 2018

Cryo-EM structure of the yeast respiratory supercomplex
Rathore S, Berndtsson J, Marin-Buera L, ..., Brzezinski P, Ott M
Nat Struct Mol Biol - (-) - [2018-12-31; online 2018-12-31]
Cryo-EM [Collaborative]
Structure of a functional obligate complex III2IV2 respiratory supercomplex from Mycobacterium smegmatis
Wiseman B, Nitharwal RG, Fedotovskaya O, ..., Brzezinski P, Högbom M
Nat Struct Mol Biol 25 (12) 1128-1136 [2018-12-00; online 2018-12-05]
Cryo-EM [Service]
The shape of the ribosome exit tunnel affects cotranslational protein folding
Kudva R, Tian P, Pardo-Avila F, ..., Bernstein HD, von Heijne G
Elife 7 (-) - [2018-11-26; online 2018-11-26]
Cryo-EM [Service]
New tools for automated high-resolution cryo-EM structure determination in RELION-3
Zivanov J, Nakane T, Forsberg BO, ..., Lindahl E, Scheres SH
Elife 7 (-) - [2018-11-09; online 2018-11-09]
Cryo-EM [Technology development]
Using Scipion for stream image processing at Cryo-EM facilities
Gómez-Blanco J, de la Rosa-Trevín JM, Marabini R, ..., Conesa P, Carazo JM
Journal of Structural Biology - (-) - [2018-10-00; online 2018-10-00]
Cryo-EM [Technology development]
The Properties of Amyloid-β Fibrils Are Determined by their Path of Formation
Brännström K, Islam T, Gharibyan AL, ..., Pamrén A, Olofsson A
Journal of Molecular Biology 430 (13) 1940-1949 [2018-06-00; online 2018-06-00]
Cryo-EM [Service]
Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen
Felten J, Vahala J, Love J, ..., Mellerowicz EJ, Sundberg B
New Phytol 218 (3) 999-1014 [2018-05-00; online 2018-03-12]
Cryo-EM [Service]
Structure of the chloroplast ribosome with chl-RRF and hibernation-promoting factor
Boerema AP, Aibara S, Paul B, ..., Lindahl E, Amunts A
PDB: 6ERI EMDB: EMD-3941 EMDB: EMD-3942 EMDB: EMD-3943
NPLANTS 4 (4) 212-217 [2018-04-00; online 2018-04-02]
Cryo-EM [Service]
Establishment of Photosynthesis through Chloroplast Development Is Controlled by Two Distinct Regulatory Phases
Dubreuil C, Jin X, Barajas-López JdD, ..., Small I, Strand Å
Plant Physiol. 176 (2) 1199-1214 [2018-02-00; online 2017-06-16]
Cryo-EM [Service]
The Folding Pathway of an Ig Domain is Conserved On and Off the Ribosome
Tian P, Steward A, Kudva R, ..., Clarke J, Best R
- - (-) - [2018-01-24; online 2018-01-24]
Cryo-EM [Service]

Year 2017

The cryo-EM structure of hibernating 100S ribosome dimer from pathogenic Staphylococcus aureus
Matzov D, Aibara S, Basu A, ..., Amunts A, Yonath AE
EMDB: EMDB-3637 EMDB: EMDB-3640 PDB: 5NG8 PDB: 5NGM
Nat Commun 8 (1) - [2017-12-00; online 2017-09-28]
Cryo-EM [Service]
Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1
Lehmann LC, Hewitt G, Aibara S, ..., Boulton SJ, Deindl S
Mendeley: https://data.mendeley.com/datasets/hm7zy8kn94/1
Molecular Cell 68 (5) 847-859.e7 [2017-12-00; online 2017-12-00]
Cryo-EM [Service] Protein Science Facility (PSF) [Service]
Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control
Carroni M, Franke KB, Maurer M, ..., Bukau B, Mogk A
EMDB: EMD-3895 EMDB: EMD-3894 EMDB: EMD-3897
Elife 6 (-) - [2017-11-22; online 2017-11-22]
Cryo-EM [Collaborative]
Cryo-EM reconstruction of the chlororibosome to 3.2 Å resolution within 24 h
Forsberg BO, Aibara S, Kimanius D, ..., Lindahl E, Amunts A
EMDB: EMD-3806 EMDB: EMD-3807 EMDB: EMD-3808
Int Union Crystallogr J IUCr J Int Union Cryst J IUCrJ 4 (6) - [2017-11-01; online 2017-09-22]
Cryo-EM [Service]
Structures of the human mitochondrial ribosome in native states of assembly
Brown A, Rathore S, Kimanius D, ..., Amunts A, Ramakrishnan V
EMDB: EMD-3842 EMDB: EMD-3843 PDB: 5OOL PDB: 5OOM
Nat Struct Mol Biol - (-) - [2017-09-11; online 2017-09-11]
Cryo-EM [Service]
The structure of the tetanus toxin reveals pH‐mediated domain dynamics
Masuyer G, Conrad J, Stenmark P
PDB: 5n0b PDB: 5n0c EMD: EMD‐3588
EMBO Rep. 18 (8) 1306-1317 [2017-08-00; online 2017-06-23]
Cryo-EM [Service]
Structural pathway of regulated substrate transfer and threading through an Hsp100 disaggregase
Deville C, Carroni M, Franke KB, ..., Mogk A, Saibil HR
EMDB: EMD-3776 EMDB: EMD-3777 PDB: 5OFO PDB: 5OG1
Sci Adv 3 (8) e1701726 [2017-08-00; online 2017-08-04]
Cryo-EM [Service]