Protein Science Facility (PSF)

Development of a chemical probe against NUDT15.

Zhang SM, Desroses M, Hagenkort A, ..., Stenmark P, Helleday T

Nat. Chem. Biol. 16 (10) 1120-1128 [2020-10-00; online 2020-07-20]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF) [Service]

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction.

Hanke L, Vidakovics Perez L, Sheward DJ, ..., Murrell B, McInerney GM

Nat Commun 11 (1) 4420 [2020-09-04; online 2020-09-04]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

Crystal structures of human PAICS reveal substrate and product binding of an emerging cancer target.

Škerlová J, Unterlass J, Göttmann M, ..., Jemth AS, Stenmark P

J. Biol. Chem. 295 (33) 11656-11668 [2020-08-14; online 2020-06-22]

A Transmembrane Crenarchaeal Mannosyltransferase Is Involved in N-Glycan Biosynthesis and Displays an Unexpected Minimal Cellulose-Synthase-like Fold.

Gandini R, Reichenbach T, Spadiut O, ..., Kalyani DC, Divne C

J. Mol. Biol. 432 (16) 4658-4672 [2020-07-24; online 2020-06-19]

Base-pair conformational switch modulates miR-34a targeting of Sirt1 mRNA.

Baronti L, Guzzetti I, Ebrahimi P, ..., Chen AA, Petzold K

Nature 583 (7814) 139-144 [2020-07-00; online 2020-05-27]

In silico Druggability Assessment of the NUDIX Hydrolase Protein Family as a Workflow for Target Prioritization.

Michel M, Homan EJ, Wiita E, ..., Helleday T, Warpman Berglund U

Front. Chem. 8 (-) 443 [2020-05-29; online 2020-05-29]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF) [Service]

MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool.

Scaletti ER, Vallin KS, Bräutigam L, ..., Stenmark P, Jemth AS

J. Biol. Chem. 295 (15) 4761-4772 [2020-04-10; online 2020-03-06]

The First Structure of an Active Mammalian dCTPase and its Complexes With Substrate Analogs and Products.

Scaletti E, Claesson M, Helleday T, Jemth AS, Stenmark P

J. Mol. Biol. 432 (4) 1126-1142 [2020-02-14; online 2020-01-15]

Year 2019

Identification of a C2-symmetric diol based human immunodeficiency virus protease inhibitor targeting Zika virus NS2B-NS3 protease.

Akaberi D, Chinthakindi PK, Båhlström A, ..., Lundkvist Å, Lennerstrand J

J. Biomol. Struct. Dyn. 38 (18) 1-11 [2019-12-27; online 2019-12-27]

Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria.

Nicholls TJ, Spåhr H, Jiang S, ..., Larsson NG, Gustafsson CM

Mol. Cell 76 (5) 784-796.e6 [2019-12-05; online 2019-10-03]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

E3 ubiquitin-protein ligase TRIM21-mediated lysine capture by UBE2E1 reveals substrate-targeting mode of a ubiquitin-conjugating E2.

Anandapadamanaban M, Kyriakidis NC, Csizmók V, ..., Wahren-Herlenius M, Sunnerhagen M

J. Biol. Chem. 294 (30) 11404-11419 [2019-07-26; online 2019-06-03]

Structural basis of inhibition of the human serine hydroxymethyltransferase SHMT2 by antifolate drugs.

Scaletti E, Jemth AS, Helleday T, Stenmark P

FEBS Lett. 593 (14) 1863-1873 [2019-07-00; online 2019-06-10]

Crystal structure of the catalytic domain of the Weissella oryzae botulinum‐like toxin

Košenina S, Masuyer G, Zhang S, Dong M, Stenmark P

FEBS Lett 593 (12) 1403-1410 [2019-06-00; online 2019-05-31]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

Crystal Structure and Substrate Specificity of the 8-oxo-dGTP Hydrolase NUDT1 from Arabidopsis thaliana.

Jemth AS, Scaletti E, Carter M, Helleday T, Stenmark P

Biochemistry 58 (7) 887-899 [2019-02-19; online 2019-01-16]

Structural Basis of Cross-Reactivity of Anti-Citrullinated Protein Antibodies.

Ge C, Xu B, Liang B, ..., Dobritzsch D, Holmdahl R

Arthritis & rheumatology (Hoboken, N.J.) 71 (2) 210-221 [2019-02-00; online 2019-01-04]

Chemical Proteomics [Collaborative] Protein Science Facility (PSF) [Service]

Year 2018

Cotranslational Folding of a Pentarepeat β-Helix Protein.

Notari L, Martínez-Carranza M, Farías-Rico JA, Stenmark P, von Heijne G

J. Mol. Biol. 430 (24) 5196-5206 [2018-12-07; online 2018-10-27]

Small-molecule inhibitor of OGG1 suppresses proinflammatory gene expression and inflammation.

Visnes T, Cázares-Körner A, Hao W, ..., Boldogh I, Helleday T

Science 362 (6416) 834-839 [2018-11-16; online 2018-11-18]

Bioinformatics Compute and Storage [Service] Chemical Biology Consortium Sweden (CBCS) [Collaborative] Chemical Proteomics [Collaborative] Drug Discovery and Development (DDD) [Service] Protein Science Facility (PSF) [Service]

MutT homologue 1 (MTH1) catalyzes the hydrolysis of mutagenic O6-methyl-dGTP.

Jemth AS, Gustafsson R, Bräutigam L, ..., Stenmark P, Helleday T

Nucleic Acids Res. 46 (20) 10888-10904 [2018-11-16; online 2018-10-12]

STAT3 differential scanning fluorimetry and differential scanning light scattering assays: Addressing a missing link in the characterization of STAT3 inhibitor interactions.

Desroses M, Busker S, Astorga-Wells J, ..., Grandér D, Page BDG

Journal of Pharmaceutical and Biomedical Analysis 160 (-) 80-88 [2018-10-25; online 2018-07-17]

Chemical Proteomics [Collaborative] Protein Science Facility (PSF) [Service]

Structural and biochemical characterization of the Cutibacterium acnes exo-β-1,4-mannosidase that targets the N-glycan core of host glycoproteins

Reichenbach T, Kalyani D, Gandini R, ..., Aspeborg H, Divne C

PLoS ONE 13 (9) e0204703 [2018-09-27; online 2018-09-27]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

14-3-3 proteins activate Pseudomonas exotoxins-S and -T by chaperoning a hydrophobic surface.

Karlberg T, Hornyak P, Pinto AF, ..., Björkegren C, Schüler H

Nat Commun 9 (1) 3785 [2018-09-17; online 2018-09-17]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

Structural and Functional Characterization of the BcsG Subunit of the Cellulose Synthase in Salmonella typhimurium.

Sun L, Vella P, Schnell R, ..., Schneider G, Römling U

J. Mol. Biol. 430 (18 Pt B) 3170-3189 [2018-09-14; online 2018-07-12]

A glutaredoxin domain fused to the radical-generating subunit of ribonucleotide reductase (RNR) functions as an efficient RNR reductant.

Rozman Grinberg I, Lundin D, Sahlin M, ..., Hofer A, Sjöberg BM

J. Biol. Chem. - (-) - [2018-08-30; online 2018-08-30]

BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence

Rosa J, Fernandez-Gonzalez E, Ducani C, Högberg B

PLoS ONE 13 (8) e0202057 [2018-08-17; online 2018-08-17]

NGI Stockholm (Genomics Applications) [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service] Protein Science Facility (PSF) [Service]

CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.

Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale J

Nat. Med. 24 (7) 927-930 [2018-07-00; online 2018-06-11]

CRISPR Functional Genomics [Collaborative] NGI Stockholm (Genomics Applications) [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service] Protein Science Facility (PSF) [Service]

Modular ssDNA binding and inhibition of telomerase activity by designer PPR proteins.

Spåhr H, Chia T, Lingford JP, ..., Filipovska A, Rackham O

Nat Commun 9 (1) 2212 [2018-06-07; online 2018-06-07]

Design, synthesis and evaluation of potent and selective inhibitors of mono-(ADP-ribosyl)transferases PARP10 and PARP14

Holechek J, Lease R, Thorsell AG, ..., Schüler H, Ferraris D

Bioorganic & Medicinal Chemistry Letters 28 (11) 2050-2054 [2018-06-00; online 2018-06-00]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

The structure of the N-terminal module of the cell wall hydrolase RipA and its role in regulating catalytic activity.

Steiner EM, Lyngsø J, Guy JE, ..., Schneider G, Schnell R

Proteins - (-) - [2018-05-02; online 2018-05-02]

Crystal Structure of Botulinum Neurotoxin A2 in Complex with the Human Protein Receptor SV2C Reveals Plasticity in Receptor Binding.

Gustafsson R, Zhang S, Masuyer G, Dong M, Stenmark P

Toxins (Basel) 10 (4) - [2018-04-12; online 2018-04-12]

Two distinct DNA sequences recognized by transcription factors represent enthalpy and entropy optima.

Morgunova E, Yin Y, Das PK, ..., Nilsson L, Taipale J

Elife 7 (-) - [2018-04-11; online 2018-04-11]

Bioinformatics Compute and Storage [Service] Protein Science Facility (PSF) [Service]

Structural and functional studies of Spr1654: an essential aminotransferase in teichoic acid biosynthesis in

Han X, Sun R, Sandalova T, Achour A

Open Biol 8 (4) - [2018-04-00; online 2018-04-20]

Identification of Poly(ADP-Ribose) Polymerase Macrodomain Inhibitors Using an AlphaScreen Protocol

Ekblad T, Verheugd P, Lindgren AE, ..., Elofsson M, Schüler H

SLAS DISCOVERY: Advancing Life Sciences R&D 23 (4) 353-362 [2018-04-00; online 2018-01-09]

Chemical Biology Consortium Sweden (CBCS) [Service] Protein Science Facility (PSF) [Collaborative]

A DHODH inhibitor increases p53 synthesis and enhances tumor cell killing by p53 degradation blockage.

Ladds MJGW, van Leeuwen IMM, Drummond CJ, ..., McCormack E, Laín S

Nat Commun 9 (1) 1107 [2018-03-16; online 2018-03-16]

Bioinformatics Compute and Storage [Service] Chemical Biology Consortium Sweden (CBCS) [Collaborative] Drug Discovery and Development (DDD) [Collaborative] Protein Science Facility (PSF) [Service]

The Immunogenicity of a Proline-Substituted Altered Peptide Ligand toward the Cancer-Associated TEIPP Neoepitope Trh4 Is Unrelated to Complex Stability.

Hafstrand I, Doorduijn EM, Sun R, ..., van Hall T, Achour A

J. Immunol. - (-) - [2018-03-05; online 2018-03-05]

Irreversible inhibition of cytosolic thioredoxin reductase 1 as a mechanistic basis for anticancer therapy.

Stafford WC, Peng X, Olofsson MH, ..., Linder S, Arnér ESJ

Sci Transl Med 10 (428) - [2018-02-14; online 2018-02-16]

Human NUDT22 Is a UDP-Glucose/Galactose Hydrolase Exhibiting a Unique Structural Fold.

Carter M, Jemth AS, Carreras-Puigvert J, ..., Helleday T, Stenmark P

Structure 26 (2) 295-303.e6 [2018-02-06; online 2018-02-08]

Crystal Structures and Inhibitor Interactions of Mouse and Dog MTH1 Reveal Species-Specific Differences in Affinity.

Narwal M, Jemth AS, Gustafsson R, ..., Helleday T, Stenmark P

Biochemistry 57 (5) 593-603 [2018-02-06; online 2018-01-16]

Novel ATP-cone-driven allosteric regulation of ribonucleotide reductase via the radical-generating subunit.

Rozman Grinberg I, Lundin D, Hasan M, ..., Logan DT, Sjöberg BM

Elife 7 (-) - [2018-02-01; online 2018-02-01]

Targeted NUDT5 inhibitors block hormone signaling in breast cancer cells.

Page BDG, Valerie NCK, Wright RHG, ..., Beato M, Helleday T

Nat Commun 9 (1) 250 [2018-01-17; online 2018-01-17]

Bioinformatics Compute and Storage [Service] Drug Discovery and Development (DDD) [Service] Protein Science Facility (PSF) [Service]

Year 2017

Structural basis for dolichylphosphate mannose biosynthesis

Gandini R, Reichenbach T, Tan TC, Divne C

Nat Commun 8 (1) - [2017-12-00; online 2017-07-25]

Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1

Lehmann LC, Hewitt G, Aibara S, ..., Boulton SJ, Deindl S

Molecular Cell 68 (5) 847-859.e7 [2017-12-00; online 2017-12-00]

Cryo-EM [Service] Protein Science Facility (PSF) [Service]

A comprehensive structural, biochemical and biological profiling of the human NUDIX hydrolase family

Carreras-Puigvert J, Zitnik M, Jemth AS, ..., Zupan B, Helleday T

Nat Commun 8 (1) - [2017-12-00; online 2017-11-16]

BioImage Informatics [Collaborative] Drug Discovery and Development (DDD) [Collaborative] Protein Science Facility (PSF) [Service]

Adipocyte Expression of SLC19A1 Links DNA Hypermethylation to Adipose Tissue Inflammation and Insulin Resistance

Petrus P, Bialesova L, Checa A, ..., Gao H, Rydén M

- - (-) - [2017-11-07; online 2017-11-07]

Combined x-ray crystallography and computational modeling approach to investigate the Hsp90 C-terminal peptide binding to FKBP51.

Kumar R, Moche M, Winblad B, Pavlov PF

Sci Rep 7 (1) 14288 [2017-10-27; online 2017-10-27]

Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX type gene cluster.

Gustafsson R, Berntsson RP, Martínez-Carranza M, ..., Johnson EA, Stenmark P

FEBS Lett. - (-) - [2017-10-25; online 2017-10-25]

Entirely enzymatic nanofabrication of DNA–protein conjugates

Bernardinelli G, Högberg B

Nucleic Acids Res 45 (18) e160-e160 [2017-10-13; online 2017-08-16]

Profiling of in vitro activities of urea-based inhibitors against cysteine synthases from Mycobacterium tuberculosis

Brunner K, Steiner EM, Reshma RS, ..., Schnell R, Schneider G

Bioorganic & Medicinal Chemistry Letters 27 (19) 4582-4587 [2017-10-00; online 2017-10-00]

Capturing LTA 4 hydrolase in action: Insights to the chemistry and dynamics of chemotactic LTB4 synthesis.

Stsiapanava A, Samuelsson B, Haeggström JZ

Proc. Natl. Acad. Sci. U.S.A. 114 (36) 9689-9694 [2017-09-05; online 2017-08-21]

Mucin-Inspired Lubrication on Hydrophobic Surfaces

Käsdorf BT, Weber F, Petrou G, ..., Crouzier T, Lieleg O

Biomacromolecules 18 (8) 2454-2462 [2017-08-14; online 2017-07-03]

Investigation of calcium-dependent activity and conformational dynamics of zebra fish 12-lipoxygenase

Mittal M, Hasan M, Balagunaseelan N, ..., Haeggström JZ, Rinaldo-Matthis A

Biochimica et Biophysica Acta (BBA) - General Subjects 1861 (8) 2099-2111 [2017-08-00; online 2017-08-00]

Biophysical characterization of the calmodulin-like domain of Plasmodium falciparum calcium dependent protein kinase 3

Andresen C, Niklasson M, Cassman Eklöf S, Wallner B, Lundström P

PLoS ONE 12 (7) e0181721 [2017-07-26; online 2017-07-26]

Protein Science Facility (PSF) [Service] Swedish NMR Centre (SNC) [Service]

Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage.

Ge C, Tong D, Liang B, ..., Dobritzsch D, Holmdahl R

JCI Insight 2 (13) - [2017-07-06; online 2017-07-06]

Autoimmunity and Serology Profiling [Service] Protein Science Facility (PSF) [Service]

Design and synthesis of potent inhibitors of the mono(ADP-ribosyl)transferase, PARP14

Upton K, Meyers M, Thorsell AG, ..., Schüler H, Ferraris D

Bioorganic & Medicinal Chemistry Letters 27 (13) 2907-2911 [2017-07-00; online 2017-07-00]

Impact of cytosine methylation on DNA binding specificities of human transcription factors

Yin Y, Morgunova E, Jolma A, ..., Vinson C, Taipale J

Science 356 (6337) eaaj2239 [2017-05-05; online 2017-05-04]

SnoN Stabilizes the SMAD3/SMAD4 Protein Complex

Walldén K, Nyman T, Hällberg BM

Sci Rep 7 (-) 46370 [2017-04-11; online 2017-04-11]

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

B. Kumar R, Zhu L, Hebert H, Jegerschöld C

J Vis Exp - (121) - [2017-03-05; online 2017-03-05]

Binding and processing of β-lactam antibiotics by the transpeptidase LdtMt2fromMycobacterium tuberculosis

Steiner EM, Schneider G, Schnell R

FEBS J 284 (5) 725-741 [2017-03-00; online 2017-02-08]

Characterization of the Ruler Protein Interaction Interface on the Substrate Specificity Switch Protein in theYersiniaType III Secretion System

Ho O, Rogne P, Edgren T, ..., Login FH, Wolf-Watz M

J. Biol. Chem. 292 (8) 3299-3311 [2017-02-24; online 2016-12-30]

Protein Science Facility (PSF) [Service] Swedish NMR Centre (SNC) [Service]

Structural Basis for Potency and Promiscuity in Poly(ADP-ribose) Polymerase (PARP) and Tankyrase Inhibitors

Thorsell AG, Ekblad T, Karlberg T, ..., Cohen MS, Schüler H

J. Med. Chem. 60 (4) 1262-1271 [2017-02-23; online 2016-12-21]

Targeting SAMHD1 with the Vpx protein to improve cytarabine therapy for hematological malignancies.

Herold N, Rudd SG, Ljungblad L, ..., Helleday T, Schaller T

Nat. Med. 23 (2) 256-263 [2017-02-00; online 2017-01-09]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF) [Service]

Small Molecule Microarray Based Discovery of PARP14 Inhibitors

Peng B, Thorsell AG, Karlberg T, Schüler H, Yao SQ

Angew. Chem. Int. Ed. 56 (1) 248-253 [2017-01-02; online 2016-12-05]

Year 2016

A ribonucleotide reductase inhibitor with deoxyribonucleoside-reversible cytotoxicity.

Crona M, Codó P, Jonna VR, ..., Fernandes AP, Tholander F

Mol Oncol 10 (9) 1375-1386 [2016-11-00; online 2016-07-26]

A novel high mobility group box 1 neutralizing chimeric antibody attenuates drug-induced liver injury and postinjury inflammation in mice.

Lundbäck P, Lea JD, Sowinska A, ..., Antoine DJ, Erlandsson Harris H

Hepatology 64 (5) 1699-1710 [2016-11-00; online 2016-09-01]

Generation and analyses of human synthetic antibody libraries and their application for protein microarrays.

Säll A, Walle M, Wingren C, ..., Borrebaeck CAK, Persson H

Protein Eng. Des. Sel. 29 (10) 427-437 [2016-10-00; online 2016-09-01]

Drug Discovery and Development (DDD) [Technology development] Protein Science Facility (PSF) [Collaborative]

Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD.

Quistgaard EM, Weininger U, Ural-Blimke Y, ..., Akke M, Löw C

BMC Biol. 14 (1) 82 [2016-09-23; online 2016-09-23]

NUDT15 Hydrolyzes 6-Thio-DeoxyGTP to Mediate the Anticancer Efficacy of 6-Thioguanine.

Valerie NC, Hagenkort A, Page BD, ..., Jemth AS, Helleday T

Cancer Res. 76 (18) 5501-5511 [2016-09-15; online 2016-08-16]

The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.

Schulte T, Mikaelsson C, Beaussart A, ..., Nygren PÅ, Achour A

Sci Rep 6 (-) 32371 [2016-09-01; online 2016-09-01]

The crystal structure of the major pneumococcal autolysin LytA in complex with a large peptidoglycan fragment reveals the pivotal role of glycans for lytic activity.

Sandalova T, Lee M, Henriques-Normark B, ..., Mellroth P, Achour A

Mol. Microbiol. 101 (6) 954-967 [2016-09-00; online 2016-07-05]

Mutation-Induced Population Shift in the MexR Conformational Ensemble Disengages DNA Binding: A Novel Mechanism for MarR Family Derepression.

Anandapadamanaban M, Pilstål R, Andresen C, ..., Wallner B, Sunnerhagen M

Structure 24 (8) 1311-1321 [2016-08-02; online 2016-07-14]

Kinetic investigation of human 5-lipoxygenase with arachidonic acid.

Mittal M, Kumar RB, Balagunaseelan N, ..., Haeggström JZ, Rinaldo-Matthis A

Bioorg. Med. Chem. Lett. 26 (15) 3547-3551 [2016-08-01; online 2016-06-11]

Inhibitors of the Cysteine Synthase CysM with Antibacterial Potency against Dormant Mycobacterium tuberculosis.

Brunner K, Maric S, Reshma RS, ..., Schnell R, Schneider G

J. Med. Chem. 59 (14) 6848-6859 [2016-07-28; online 2016-07-13]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF) [Service]

Identification of Inhibitors of Pseudomonas aeruginosa Exotoxin-S ADP-Ribosyltransferase Activity.

Pinto AF, Ebrahimi M, Saleeb M, ..., Elofsson M, Schüler H

J Biomol Screen 21 (6) 590-595 [2016-07-00; online 2016-02-05]

Combined structural, biochemical and cellular evidence demonstrates that both FGDF motifs in alphavirus nsP3 are required for efficient replication.

Schulte T, Liu L, Panas MD, ..., Achour A, McInerney GM

Open Biol 6 (7) - [2016-07-00; online 2016-07-08]

Coxsackievirus counters the host innate immune response by blocking type III interferon expression.

Lind K, Svedin E, Domsgen E, ..., Moll M, Flodström-Tullberg M

J. Gen. Virol. 97 (6) 1-12 [2016-06-00; online 2016-03-02]

Divergent non-heme iron enzymes in the nogalamycin biosynthetic pathway.

Siitonen V, Selvaraj B, Niiranen L, ..., Schneider G, Metsä-Ketelä M

Proc. Natl. Acad. Sci. U.S.A. 113 (19) 5251-5256 [2016-05-10; online 2016-04-25]

Sister Chromatid Cohesion Establishment Factor ESCO1 Operates by Substrate-Assisted Catalysis.

Kouznetsova E, Kanno T, Karlberg T, ..., Sjögren C, Schüler H

Structure 24 (5) 789-796 [2016-05-03; online 2016-04-21]

Novel mode of inhibition by D-tagatose 6-phosphate through a Heyns rearrangement in the active site of transaldolase B variants.

Stellmacher L, Sandalova T, Schneider S, ..., Sprenger GA, Samland AK

Acta Crystallogr D Struct Biol 72 (Pt 4) 467-476 [2016-04-00; online 2016-03-24]

Engineering a thermostable Halothermothrix orenii β-glucosidase for improved galacto-oligosaccharide synthesis.

Hassan N, Geiger B, Gandini R, ..., Divne C, Tan TC

Appl. Microbiol. Biotechnol. 100 (8) 3533-3543 [2016-04-00; online 2015-12-02]

A saposin-lipoprotein nanoparticle system for membrane proteins.

Frauenfeld J, Löving R, Armache JP, ..., Cheng Y, Nordlund P

Nat. Methods 13 (4) 345-351 [2016-04-00; online 2016-03-07]

CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.

Almqvist H, Axelsson H, Jafari R, ..., Lundbäck T, Nordlund P

Nat Commun 7 (-) 11040 [2016-03-24; online 2016-03-24]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF) [Service]

Crystal Structures of Type-II Inositol Polyphosphate 5-Phosphatase INPP5B with Synthetic Inositol Polyphosphate Surrogates Reveal New Mechanistic Insights for the Inositol 5-Phosphatase Family.

Mills SJ, Silvander C, Cozier G, ..., Nordlund P, Potter BV

Biochemistry 55 (9) 1384-1397 [2016-03-08; online 2016-02-29]

The MHC Class I Cancer-Associated Neoepitope Trh4 Linked with Impaired Peptide Processing Induces a Unique Noncanonical TCR Conformer.

Hafstrand I, Doorduijn EM, Duru AD, ..., van Hall T, Achour A

J. Immunol. 196 (5) 2327-2334 [2016-03-01; online 2016-01-22]

The structure and catalytic mechanism of human sphingomyelin phosphodiesterase like 3a--an acid sphingomyelinase homologue with a novel nucleotide hydrolase activity.

Lim SM, Yeung K, Trésaugues L, Ling TH, Nordlund P

FEBS J. 283 (6) 1107-1123 [2016-03-00; online 2016-02-05]

Discovery of the First Potent and Selective Inhibitors of Human dCTP Pyrophosphatase 1.

Llona-Minguez S, Höglund A, Jacques SA, ..., Scobie M, Helleday T

J. Med. Chem. 59 (3) 1140-1148 [2016-02-11; online 2016-01-27]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Drug Discovery and Development (DDD) [Collaborative] Protein Science Facility (PSF) [Service]

Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa.

Jacewicz A, Schnell R, Lindqvist Y, Schneider G

Acta Crystallogr F Struct Biol Commun 72 (Pt 2) 105-111 [2016-02-00; online 2016-01-22]

A dynamic Asp-Arg interaction is essential for catalysis in microsomal prostaglandin E2 synthase.

Brock JS, Hamberg M, Balagunaseelan N, ..., Rinaldo-Matthis A, Haeggström JZ

Proc. Natl. Acad. Sci. U.S.A. 113 (4) 972-977 [2016-01-26; online 2016-01-11]

Year 2015

Exploring water as building bricks in enzyme engineering.

Hendil-Forssell P, Martinelle M, Syrén PO

Chem. Commun. (Camb.) 51 (97) 17221-17224 [2015-12-18; online 2015-10-02]

Structural insights into the DNA-binding specificity of E2F family transcription factors.

Morgunova E, Yin Y, Jolma A, ..., Nilsson L, Taipale J

Nat Commun 6 (1) 10050 [2015-12-03; online 2015-12-03]

Karolinska High Throughput Center (KHTC) Protein Science Facility (PSF)

DNA-dependent formation of transcription factor pairs alters their binding specificity.

Jolma A, Yin Y, Nitta KR, ..., Morgunova E, Taipale J

Nature 527 (7578) 384-388 [2015-11-19; online 2015-11-10]

Karolinska High Throughput Center (KHTC) Protein Science Facility (PSF)

Trimeric microsomal glutathione transferase 2 displays one third of the sites reactivity.

Ahmad S, Thulasingam M, Palombo I, ..., Haeggström JZ, Rinaldo-Matthis A

Biochim. Biophys. Acta 1854 (10 Pt A) 1365-1371 [2015-10-00; online 2015-06-13]

Acid-Base Catalyst Discriminates between a Fructose 6-Phosphate Aldolase and a Transaldolase

DOI Crossref

Stellmacher L, Sandalova T, Leptihn S, ..., Sprenger GA, Samland AK

ChemCatChem 7 (19) 3140-3151 [2015-10-00; online 2015-08-06]

Half-of-the-Sites Reactivity of the Castor Δ9-18:0-Acyl Carrier Protein Desaturase.

Liu Q, Chai J, Moche M, ..., Lindqvist Y, Shanklin J

Plant Physiol. 169 (1) 432-441 [2015-09-00; online 2015-08-01]

Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.

Possner DD, Claesson M, Guy JE

PLoS ONE 10 (8) e0136239 [2015-08-21; online 2015-08-21]

Crystal structure, biochemical and cellular activities demonstrate separate functions of MTH1 and MTH2.

Carter M, Jemth AS, Hagenkort A, ..., Helleday T, Stenmark P

Nat Commun 6 (-) 7871 [2015-08-04; online 2015-08-04]

Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation.

Tan TC, Kracher D, Gandini R, ..., Ludwig R, Divne C

Nat Commun 6 (-) 7542 [2015-07-07; online 2015-07-07]

Structural Basis for the Specificity of Human NUDT16 and Its Regulation by Inosine Monophosphate

Trésaugues L, Lundbäck T, Welin M, ..., Gräslund S, Nordlund P

PLoS ONE 10 (6) e0131507 [2015-06-29; online 2015-06-29]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF)

Structure of the nuclease subunit of human mitochondrial RNase P.

Reinhard L, Sridhara S, Hällberg BM

Nucleic Acids Res. 43 (11) 5664-5672 [2015-06-23; online 2015-05-09]

Crystal structures of the kinase domain of the sulfate-activating complex in Mycobacterium tuberculosis.

Poyraz Ö, Brunner K, Lohkamp B, ..., Schnell R, Schneider G

PLoS ONE 10 (3) e0121494 [2015-03-25; online 2015-03-25]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Protein Science Facility (PSF)

Structural basis for lack of ADP-ribosyltransferase activity in poly(ADP-ribose) polymerase-13/zinc finger antiviral protein.

Karlberg T, Klepsch M, Thorsell AG, ..., Linusson A, Schüler H

J. Biol. Chem. 290 (12) 7336-7344 [2015-03-20; online 2015-01-31]

Structural characterization of substrate and inhibitor binding to farnesyl pyrophosphate synthase from Pseudomonas aeruginosa.

Schmidberger JW, Schnell R, Schneider G

Acta Crystallogr. D Biol. Crystallogr. 71 (Pt 3) 721-731 [2015-03-00; online 2015-02-26]

High-resolution crystal structure of a polyextreme GH43 glycosidase from Halothermothrix orenii with α-L-arabinofuranosidase activity.

Hassan N, Kori LD, Gandini R, ..., Divne C, Tan TC

Acta Crystallogr F Struct Biol Commun 71 (Pt 3) 338-345 [2015-03-00; online 2015-02-19]

Biochemical and structural characterization of a thermostable β-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.

Hassan N, Nguyen TH, Intanon M, ..., Divne C, Tan TC

Appl. Microbiol. Biotechnol. 99 (4) 1731-1744 [2015-02-00; online 2014-09-01]

Year 2014

A novel cytosolic NADH:quinone oxidoreductase fromMethanothermobacter marburgensis

Ullmann E, Tan TC, Gundinger T, ..., Divne C, Spadiut O

Biosci. Rep. 34 (6) 893-904 [2014-12-23; online 2014-12-23]

Structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme.

Dobritzsch D, Wang H, Schneider G, Yu S

Biochem. J. 462 (3) 441-452 [2014-09-15; online 2014-06-21]

Structural basis for regulation of the human acetyl-CoA thioesterase 12 and interactions with the steroidogenic acute regulatory protein-related lipid transfer (START) domain.

Swarbrick CM, Roman N, Cowieson N, ..., Lehtiö L, Forwood JK

J. Biol. Chem. 289 (35) 24263-24274 [2014-08-29; online 2014-07-09]

Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2.

Guettou F, Quistgaard EM, Raba M, ..., Löw C, Nordlund P

Nat. Struct. Mol. Biol. 21 (8) 728-731 [2014-08-00; online 2014-07-30]

Comparative structural analysis of the putative mono-ADP-ribosyltransferases of the ARTD/PARP family.

Pinto AF, Schüler H

Curr. Top. Microbiol. Immunol. 384 (-) 153-166 [2014-07-13; online 2014-07-13]

Structural and biochemical characterization of human PR70 in isolation and in complex with the scaffolding subunit of protein phosphatase 2A.

Dovega R, Tsutakawa S, Quistgaard EM, ..., Löw C, Nordlund P

PLoS ONE 9 (7) e101846 [2014-07-09; online 2014-07-09]

Structural basis for PTPA interaction with the invariant C-terminal tail of PP2A.

Löw C, Quistgaard EM, Kovermann M, ..., Balbach J, Nordlund P

Biol. Chem. 395 (7-8) 881-889 [2014-07-00; online 2014-07-09]

Crystal structure of human CRMP-4: correction of intensities for lattice-translocation disorder

Ponnusamy R, Lebedev AA, Pahlow S, Lohkamp B

Acta Crystallogr. D Biol. Crystallogr. 70 (6) 1680-1694 [2014-06-01; online 2014-05-30]

Structure and inhibition of mouse leukotriene C4 synthase.

Niegowski D, Kleinschmidt T, Ahmad S, ..., Rinaldo-Matthis A, Haeggström JZ

PLoS ONE 9 (5) e96763 [2014-05-08; online 2014-05-08]

Chemical Biology Consortium Sweden (CBCS) [Service] Protein Science Facility (PSF)

Structural basis for phosphoinositide substrate recognition, catalysis, and membrane interactions in human inositol polyphosphate 5-phosphatases.

Trésaugues L, Silvander C, Flodin S, ..., Berglund H, Nordlund P

Structure 22 (5) 744-755 [2014-05-06; online 2014-04-08]

MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool.

Gad H, Koolmeister T, Jemth A, ..., Berglund UW, Helleday T

Nature 508 (7495) 215-221 [2014-04-10; online 2014-04-02]

Chemical Biology Consortium Sweden (CBCS) [Collaborative] Drug Discovery and Development (DDD) [Collaborative] Protein Science Facility (PSF)

Design, synthesis, crystallographic studies, and preliminary biological appraisal of new substituted triazolo[4,3-b]pyridazin-8-amine derivatives as tankyrase inhibitors.

Liscio P, Carotti A, Asciutti S, ..., Pellicciari R, Camaioni E

J. Med. Chem. 57 (6) 2807-2812 [2014-03-27; online 2014-02-18]

Binding of Pro-Gly-Pro at the active site of leukotriene A4 hydrolase/aminopeptidase and development of an epoxide hydrolase selective inhibitor.

Stsiapanava A, Olsson U, Wan M, ..., Rinaldo-Matthis A, Haeggström JZ

Proc. Natl. Acad. Sci. U.S.A. 111 (11) 4227-4232 [2014-03-18; online 2014-03-05]

Advanced Mass Spectrometry Proteomics Protein Science Facility (PSF)

Epitope-specific antibody response is controlled by immunoglobulin V(H) polymorphisms.

Raposo B, Dobritzsch D, Ge C, ..., Schneider G, Holmdahl R

J. Exp. Med. 211 (3) 405-411 [2014-03-10; online 2014-02-19]

ZapE is a novel cell division protein interacting with FtsZ and modulating the Z-ring dynamics.

Marteyn BS, Karimova G, Fenton AK, ..., Sansonetti PJ, Tang CM

MBio 5 (2) e00022-e00014 [2014-03-04; online 2014-03-04]

Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.

Niegowski D, Kleinschmidt T, Olsson U, ..., Rinaldo-Matthis A, Haeggström JZ

J. Biol. Chem. 289 (8) 5199-5207 [2014-02-21; online 2013-12-25]

Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae.

Mellroth P, Sandalova T, Kikhney A, ..., Henriques-Normark B, Achour A

MBio 5 (1) e01120-e01113 [2014-02-11; online 2014-02-11]

Product formation controlled by substrate dynamics in leukotriene A4 hydrolase.

Stsiapanava A, Tholander F, Kumar RB, ..., Haeggström JZ, Rinaldo-Matthis A

Biochim. Biophys. Acta 1844 (2) 439-446 [2014-02-00; online 2013-12-18]

Structural basis for binding of fluorinated glucose and galactose to Trametes multicolor pyranose 2-oxidase variants with improved galactose conversion.

Tan TC, Spadiut O, Gandini R, Haltrich D, Divne C

PLoS ONE 9 (1) e86736 [2014-01-21; online 2014-01-21]

The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.

Schulte T, Löfling J, Mikaelsson C, ..., Henriques-Normark B, Achour A

Open Biol 4 (-) 130090 [2014-01-15; online 2014-01-15]

RipD (Rv1566c) from Mycobacterium tuberculosis: adaptation of an NlpC/p60 domain to a non-catalytic peptidoglycan-binding function.

Böth D, Steiner EM, Izumi A, Schneider G, Schnell R

Biochem. J. 457 (1) 33-41 [2014-01-01; online 2013-10-11]

Year 2013

Chemical probes to study ADP-ribosylation: synthesis and biochemical evaluation of inhibitors of the human ADP-ribosyltransferase ARTD3/PARP3.

Lindgren AE, Karlberg T, Ekblad T, ..., Schüler H, Elofsson M

J. Med. Chem. 56 (23) 9556-9568 [2013-12-12; online 2013-11-06]

Substrate specificity and oligomerization of human GMP synthetase.

Welin M, Lehtiö L, Johansson A, ..., Gräslund S, Nordlund P

J. Mol. Biol. 425 (22) 4323-4333 [2013-11-15; online 2013-07-03]

Discovery of an allosteric inhibitor binding site in 3-Oxo-acyl-ACP reductase from Pseudomonas aeruginosa.

Cukier CD, Hope AG, Elamin AA, ..., Gray DW, Schneider G

ACS Chem. Biol. 8 (11) 2518-2527 [2013-11-15; online 2013-09-11]

Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms.

Quistgaard EM, Löw C, Moberg P, Nordlund P

J. Struct. Biol. 184 (2) 375-378 [2013-11-00; online 2013-09-26]

Nanobody mediated crystallization of an archeal mechanosensitive channel.

Löw C, Yau YH, Pardon E, ..., Steyaert J, Nordlund P

PLoS ONE 8 (10) e77984 [2013-10-21; online 2013-10-21]

Structural insights into substrate recognition in proton-dependent oligopeptide transporters.

Guettou F, Quistgaard EM, Trésaugues L, ..., Nordlund P, Löw C

EMBO Rep. 14 (9) 804-810 [2013-09-00; online 2013-07-23]

PARP inhibitor with selectivity toward ADP-ribosyltransferase ARTD3/PARP3.

Lindgren AE, Karlberg T, Thorsell AG, ..., Elofsson M, Schüler H

ACS Chem. Biol. 8 (8) 1698-1703 [2013-08-16; online 2013-06-08]

Structural and biophysical characterization of the cytoplasmic domains of human BAP29 and BAP31.

Quistgaard EM, Löw C, Moberg P, ..., Maddi K, Nordlund P

PLoS ONE 8 (8) e71111 [2013-08-13; online 2013-08-13]

Advanced Mass Spectrometry Proteomics Protein Science Facility (PSF)

High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation.

Anandapadamanaban M, Andresen C, Helander S, ..., Moche M, Sunnerhagen M

Nat. Struct. Mol. Biol. 20 (8) 1008-1014 [2013-08-00; online 2013-07-16]

Enzymatic production of 'monoclonal stoichiometric' single-stranded DNA oligonucleotides.

Ducani C, Kaul C, Moche M, Shih WM, Högberg B

Nat. Methods 10 (7) 647-652 [2013-07-00; online 2013-06-04]

The crystal structure of the lumenal domain of Erv41p, a protein involved in transport between the endoplasmic reticulum and Golgi apparatus.

Biterova EI, Svärd M, Possner DD, Guy JE

J. Mol. Biol. 425 (12) 2208-2218 [2013-06-26; online 2013-03-26]

Substrate Channel Flexibility in Pseudomonas aeruginosa MurB Accommodates Two Distinct Substrates.

Chen MW, Lohkamp B, Schnell R, Lescar J, Schneider G

PLoS ONE 8 (6) e66936 [2013-06-21; online 2013-06-21]

Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters.

Quistgaard EM, Löw C, Moberg P, Trésaugues L, Nordlund P

Nat. Struct. Mol. Biol. 20 (6) 766-768 [2013-06-00; online 2013-04-30]

Structural and dynamic insights into substrate binding and catalysis of human lipocalin prostaglandin D synthase.

Lim SM, Chen D, Teo H, ..., Pervushin K, Nordlund P

J. Lipid Res. 54 (6) 1630-1643 [2013-06-00; online 2013-03-26]

Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains.

Forst AH, Karlberg T, Herzog N, ..., Schüler H, Lüscher B

Structure 21 (3) 462-475 [2013-03-05; online 2013-03-12]

Structure of LdtMt2, an L,D-transpeptidase from Mycobacterium tuberculosis.

Böth D, Steiner EM, Stadler D, ..., Schnell R, Schneider G

Acta Crystallogr. D Biol. Crystallogr. 69 (Pt 3) 432-441 [2013-03-00; online 2013-02-16]

Year 2012

Unexpected T-cell recognition of an altered peptide ligand is driven by reversed thermodynamics.

Allerbring EB, Duru AD, Uchtenhagen H, ..., Nygren PÅ, Achour A

Eur. J. Immunol. 42 (11) 2990-3000 [2012-11-00; online 2012-07-28]

High-resolution insights into binding of unfolded polypeptides by the PPIase chaperone SlpA.

Quistgaard EM, Nordlund P, Löw C

FASEB J. 26 (10) 4003-4013 [2012-10-00; online 2012-06-28]

Discovery of Ligands for ADP-Ribosyltransferases via Docking-Based Virtual Screening

Andersson CD, Karlberg T, Ekblad T, ..., Schüler H, Linusson A

J. Med. Chem. 55 (17) 7706-7718 [2012-09-13; online 2012-09-13]

Chemical Biology Consortium Sweden (CBCS) [Service] Protein Science Facility (PSF)

Inflammation-associated nitrotyrosination affects TCR recognition through reduced stability and alteration of the molecular surface of the MHC complex.

Madhurantakam C, Duru AD, Sandalova T, Webb JR, Achour A

PLoS ONE 7 (3) e32805 [2012-03-14; online 2012-03-14]

Tetrahydrodipicolinate N-succinyltransferase and dihydrodipicolinate synthase from Pseudomonas aeruginosa: structure analysis and gene deletion.

Schnell R, Oehlmann W, Sandalova T, ..., Singh M, Schneider G

PLoS ONE 7 (2) e31133 [2012-02-16; online 2012-02-16]

High-resolution structure of a BRICHOS domain and its implications for anti-amyloid chaperone activity on lung surfactant protein C.

Willander H, Askarieh G, Landreh M, ..., Knight SD, Johansson J

Proc. Natl. Acad. Sci. U.S.A. 109 (7) 2325-2329 [2012-02-14; online 2012-02-07]