High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction.

Graça AT, Hall M, Persson K, Schröder WP

Sci Rep 11 (1) 15534 [2021-07-30; online 2021-07-30]

In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 Å, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated Mn4CaO5-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation.

Cryo-EM [Collaborative]

PubMed 34330992

DOI 10.1038/s41598-021-94914-x

Crossref 10.1038/s41598-021-94914-x

pii: 10.1038/s41598-021-94914-x
pmc: PMC8324835