Using RELION software within the Scipion framework.

Sharov G, Morado DR, Carroni M, de la Rosa-TrevĂ­n JM

Acta Crystallogr D Struct Biol 77 (4) 403-410 [2021-04-01; online 2021-03-30]

Scipion is a modular image-processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrated with an image-processing pipeline for published data. The user interfaces of Scipion and RELION are compared and the key differences are highlighted, allowing this manuscript to be used as a guide for both new and experienced users of this software. Different on-the-fly image-processing options are also discussed, demonstrating the flexibility of the Scipion framework.

Cryo-EM [Technology development]

PubMed 33825701

DOI 10.1107/S2059798321001856

Crossref 10.1107/S2059798321001856

pii: S2059798321001856
pmc: PMC8025880

Publications 9.5.0