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Hussein R, Graça A, Forsman J, Aydin AO, Hall M, Gaetcke J, Chernev P, Wendler P, Dobbek H, Messinger J, Zouni A, Schröder WP
Science 384 (6702) 1349-1355 [2024-06-21; online 2024-06-20]
Photosystem II starts the photosynthetic electron transport chain that converts solar energy into chemical energy and thus sustains life on Earth. It catalyzes two chemical reactions: water oxidation to molecular oxygen and plastoquinone reduction. Coupling of electron and proton transfer is crucial for efficiency; however, the molecular basis of these processes remains speculative owing to uncertain water binding sites and the lack of experimentally determined hydrogen positions. We thus collected high-resolution cryo-electron microscopy data of fully hydrated photosystem II from the thermophilic cyanobacterium Thermosynechococcus vestitus to a final resolution of 1.71 angstroms. The structure reveals several previously undetected partially occupied water binding sites and more than half of the hydrogen and proton positions. This clarifies the pathways of substrate water binding and plastoquinone B protonation.
PubMed 38900892
DOI 10.1126/science.adn6541
Crossref 10.1126/science.adn6541