Cryo-EM structure of the yeast respiratory supercomplex.

Rathore S, Berndtsson J, Marin-Buera L, Conrad J, Carroni M, Brzezinski P, Ott M

Nat. Struct. Mol. Biol. 26 (1) 50-57 [2019-01-00; online 2018-12-31]

Respiratory chain complexes execute energy conversion by connecting electron transport with proton translocation over the inner mitochondrial membrane to fuel ATP synthesis. Notably, these complexes form multi-enzyme assemblies known as respiratory supercomplexes. Here we used single-particle cryo-EM to determine the structures of the yeast mitochondrial respiratory supercomplexes III 2IV and III2IV2, at 3.2-Å and 3.5-Å resolutions, respectively. We revealed the overall architecture of the supercomplex, which deviates from the previously determined assemblies in mammals; obtained a near-atomic structure of the yeast complex IV; and identified the protein-protein and protein-lipid interactions implicated in supercomplex formation. Take together, our results demonstrate convergent evolution of supercomplexes in mitochondria that, while building similar assemblies, results in substantially different arrangements and structural solutions to support energy conversion.

Cryo-EM [Collaborative]

PubMed 30598556

DOI 10.1038/s41594-018-0169-7

Crossref 10.1038/s41594-018-0169-7

pii: 10.1038/s41594-018-0169-7

Publications 9.5.0