CRISPR Functional Genomics

LRPPRC and SLIRP synergize to maintain sufficient and orderly mammalian mitochondrial translation.

Rubalcava-Gracia D, Bubb K, Levander F, ..., Koolmeister C, Larsson NG

Nucleic Acids Res. 52 (18) 11266-11282 [2024-10-14; online 2024-08-01]

Bioinformatics Support and Infrastructure [Collaborative] Bioinformatics Support, Infrastructure and Training [Collaborative] CRISPR Functional Genomics [Service]

Epigenetic regulation of cell state by H2AFY governs immunogenicity in high-risk neuroblastoma.

Nagarajan D, Parracho RT, Corujo D, ..., Buschbeck M, Mao Y

J. Clin. Invest. 134 (21) - [2024-09-10; online 2024-09-10]

CRISPR-Cas9 Knockout Screens Identify DNA Damage Response Pathways and BTK as Essential for Cisplatin Response in Diffuse Large B-Cell Lymphoma

Issa II, Due H, Brøndum RF, ..., Schmierer B, Dybkær K

Cancers (Basel) 16 (13) 2437 [2024-07-02; online 2024-07-02]

Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer.

Papakyriacou I, Kutkaite G, Rúbies Bedós M, ..., Menden MP, Mao Y

Nat Commun 15 (1) 3581 [2024-04-27; online 2024-04-27]

Radiolabeled 15-mer peptide internalization is mediated by megalin (LRP2 receptor) in a CRISPR/Cas9-based LRP2 knockout human kidney cell model.

Durinova A, Smutna L, Barta P, ..., Pavek P, Trejtnar F

EJNMMI Radiopharm Chem 9 (1) 32 [2024-04-18; online 2024-04-18]

Deletion of the TMEM30A gene enables leukemic cell evasion of NK cell cytotoxicity.

Kristenson L, Badami C, Ljungberg A, ..., Tang KW, Thorén FB

Proc. Natl. Acad. Sci. U.S.A. 121 (15) e2316447121 [2024-04-09; online 2024-04-01]

Abstract 2025: Zeb1 downregulation sensitizes pancreatic cancer-associated fibroblasts to killing by oncolytic reovirus through upregulation of the reovirus receptor junction adhesion molecule A

Dam N, Harryvan TJ, Schmierer B, Hawinkels LJAC, Kemp V

Cancer Res. 84 (6_Supplement) 2025-2025 [2024-03-22; online 2024-03-22]

A distinctive tumor compartment in pancreatic lobules defined by nascent stroma and classical tumor cell phenotype

Söderqvist S, Viljamaa A, Geyer N, ..., Moro CF, Gerling M

- - (-) - [2024-03-15; online 2024-03-15]

Year 2023

An idiosyncratic zonated stroma encapsulates desmoplastic liver metastases and originates from injured liver.

Fernández Moro C, Geyer N, Harrizi S, ..., Bozóky B, Gerling M

Nat Commun 14 (1) 5024 [2023-08-18; online 2023-08-18]

CRISPR Functional Genomics [Service] NGI Short read [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service]

A large-scale sORF screen identifies putative microproteins and provides insights into their interaction partners, localisation and function

Schlesinger D, Dirks C, Luzon CN, ..., Eirich J, Elsässer SJ

- - (-) - [2023-06-13; online 2023-06-13]

Bioinformatics Support for Computational Resources [Service] CRISPR Functional Genomics [Service]

Investigation of enzalutamide, docetaxel, and cabazitaxel resistance in the castration resistant prostate cancer cell line C4 using genome-wide CRISPR/Cas9 screening.

Haldrup J, Weiss S, Schmidt L, Sørensen KD

Sci Rep 13 (1) 9043 [2023-06-03; online 2023-06-03]

CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma.

Wenger A, Karlsson I, Kling T, Carén H

Clin Epigenetics 15 (1) 80 [2023-05-09; online 2023-05-09]

CRISPR Functional Genomics [Service] Integrated Microscopy Technologies Gothenburg [Service]

Abstract 3778: Genome-Scale CRISPRa and CRISPRi screening for lncRNA drivers of prostate cancer progression

Weiss S, Lord A, Schmierer B, ..., Borre M, Sørensen KD

Cancer Res. 83 (7_Supplement) 3778-3778 [2023-04-04; online 2023-04-04]

Year 2022

Abstract A007: Pancreatic cancer comprises co-existing transcriptional states regulated by distinct master regulator programs

Laise P, Turunen M, Maurer HC, ..., Olive KPP, Califano A

Cancer Res. 82 (22_Supplement) A007-A007 [2022-11-15; online 2022-11-15]

New regulators of the tetracycline‐inducible gene expression system identified by chemical and genetic screens

Colicchia V, Häggblad M, Sirozh O, ..., Hühn D, Fernandez‐Capetillo O

FEBS Open Bio 12 (10) 1896-1908 [2022-10-00; online 2022-09-11]

CRISPR Functional Genomics [Service] Chemical Biology Consortium Sweden (CBCS) [Service]

MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".

Das SK, Kuzin V, Cameron DP, ..., Levens D, Baranello L

Mol. Cell 82 (1) 140-158.e12 [2022-01-06; online 2021-12-09]

Metabolic resistance to the inhibition of mitochondrial transcription revealed by CRISPR-Cas9 screen.

Mennuni M, Filograna R, Felser A, ..., Lytovchenko O, Larsson N

EMBO Rep. 23 (1) e53054 [2022-01-05; online 2021-11-15]

CRISPR Functional Genomics [Service] NGI Short read [Service] National Genomics Infrastructure [Service]

Year 2021

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

Reint G, Li Z, Labun K, ..., Taipale J, Haapaniemi E

Elife 10 (-) - [2021-12-13; online 2021-12-13] Open Access

Inhibition of the ubiquitin-proteasome system by an NQO1-activatable compound.

Giovannucci TA, Salomons FA, Haraldsson M, ..., Johnsen JI, Dantuma NP

Cell Death Dis 12 (10) 914 [2021-10-06; online 2021-10-06]

CRISPR Functional Genomics [Service] Chemical Biology Consortium Sweden (CBCS) [Collaborative] Global Proteomics and Proteogenomics [Service] NGI Stockholm (Genomics Applications) [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service]

Year 2020

Developmental and MAPK-responsive transcription factors drive distinct malignant subtypes and genetic dependencies in pancreatic cancer

Laise P, Turunen M, Garcia AC, ..., Olive KP, Califano A

- - (-) - [2020-10-27; online 2020-10-27]

Designing custom CRISPR libraries for hypothesis-driven drug target discovery.

Iyer VS, Jiang L, Shen Y, ..., Schmierer B, Wermeling F

Computational and Structural Biotechnology Journal 18 (-) 2237-2246 [2020-08-18; online 2020-08-18]

Bioinformatics Support for Computational Resources [Service] CRISPR Functional Genomics [Collaborative]

A regulatory role for CHD2 in myelopoiesis.

Shahin Varnoosfaderani F, Palau A, Dong W, ..., Svensson JP, Lennartsson A

Epigenetics 15 (6-7) 702-714 [2020-01-10; online 2020-01-10]

Bioinformatics Support for Computational Resources [Service] CRISPR Functional Genomics [Service] NGI Stockholm (Genomics Applications) [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service]

Year 2019

Reply to "CRISPR screens are feasible in TP53 wild-type cells".

Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale J

Mol. Syst. Biol. 15 (8) e9059 [2019-08-00; online 2019-08-30]

TGF-β family ligands exhibit distinct signalling dynamics that are driven by receptor localisation.

Miller DSJ, Schmierer B, Hill CS

J. Cell. Sci. 132 (14) - [2019-07-15; online 2019-07-15]

Year 2018

Contribution of allelic imbalance to colorectal cancer.

Palin K, Pitkänen E, Turunen M, ..., Taipale J, Aaltonen LA

Nat Commun 9 (1) 3664 [2018-09-10; online 2018-09-10]

Bioinformatics Support for Computational Resources [Service] CRISPR Functional Genomics [Collaborative] NGI Stockholm (Genomics Applications) [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service]

CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.

Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale J

Nat. Med. 24 (7) 927-930 [2018-07-00; online 2018-06-11]

CRISPR Functional Genomics [Collaborative] NGI Stockholm (Genomics Applications) [Service] NGI Stockholm (Genomics Production) [Service] National Genomics Infrastructure [Service] Protein Science Facility (PSF) [Service]

Year 2017