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Schramm A, Lieutaud P, Gianni S, Longhi S, Bignon C
Int J Mol Sci 19 (1) - [2017-12-29; online 2017-12-29]
InSiDDe (In Silico Disorder Design) is a program for the in silico design of intrinsically disordered proteins of desired length and disorder probability. The latter is assessed using IUPred and spans values ranging from 0.55 to 0.95 with 0.05 increments. One to ten artificial sequences per query, each made of 50 to 200 residues, can be generated by InSiDDe. We describe the rationale used to set up InSiDDe and show that an artificial sequence of 100 residues with an IUPred score of 0.6 designed by InSiDDe could be recombinantly expressed in E. coli at high levels without degradation when fused to a natural molecular recognition element (MoRE). In addition, the artificial fusion protein exhibited the expected behavior in terms of binding modulation of the specific partner recognized by the MoRE. To the best of our knowledge, InSiDDe is the first publicly available software for the design of intrinsically disordered protein (IDP) sequences. InSiDDE is publicly available online.
Protein Science Facility (PSF) [Service]
PubMed 29286306
DOI 10.3390/ijms19010091
Crossref 10.3390/ijms19010091
pmc: PMC5796041
pii: ijms19010091