Sister Chromatid Cohesion Establishment Factor ESCO1 Operates by Substrate-Assisted Catalysis.

Kouznetsova E, Kanno T, Karlberg T, Thorsell AG, Wisniewska M, Kursula P, Sjögren C, Schüler H

Structure 24 (5) 789-796 [2016-05-03; online 2016-04-21]

Sister chromatid cohesion, formed by the cohesin protein complex, is essential for chromosome segregation. In order for cohesion to be established, the cohesin subunit SMC3 needs to be acetylated by a homolog of the ESCO1/Eco1 acetyltransferases, the enzymatic mechanism of which has remained unknown. Here we report the crystal structure of the ESCO1 acetyltransferase domain in complex with acetyl-coenzyme A, and show by SAXS that ESCO1 is a dimer in solution. The structure reveals an active site that lacks a potential catalytic base side chain. However, mutation of glutamate 789, a surface residue that is close to the automodification target lysine 803, strongly reduces autoacetylation of ESCO1. Moreover, budding yeast Smc3 mutated at the conserved residue D114, adjacent to the cohesion-activating acetylation site K112,K113, cannot be acetylated in vivo. This indicates that ESCO1 controls cohesion through substrate-assisted catalysis. Thus, this study discloses a key mechanism for cohesion establishment.

Protein Science Facility (PSF) [Service]

PubMed 27112597

DOI 10.1016/j.str.2016.03.021

Crossref 10.1016/j.str.2016.03.021

pii: S0969-2126(16)30032-6

Publications 9.5.0