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Henkel E, Li Z, Uvehag D, Schmierer B, Henkel M, Wermeling F
CRISPR J - (-) - [2025-05-07; online 2025-05-07]
Custom CRISPR screens are powerful tools for rapid, hypothesis-driven discovery, but their design is often complex and time-consuming. Green Listed v2.0 simplifies this process with an intuitive workflow for designing custom CRISPR spacer libraries and supports downstream analysis for all users, irrespective of their computational experience. The web application features a user-friendly graphical interface freely accessible at https://greenlisted.cmm.se. Version 2.0 includes significant upgrades to the original 2016 version that were implemented based on user feedback. This includes a new gene synonym tool, expanded library options, optimized output lists, performance improvements, and linked scripts for the rational design of custom CRISPR screen gene sets.
CRISPR Functional Genomics [Technology development]
PubMed 40329823
DOI 10.1089/crispr.2025.0023
Crossref 10.1089/crispr.2025.0023