High-Density Antigen Microarrays for the Assessment of Antibody Selectivity and Off-Target Binding.

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Sjöberg R, Andersson E, Hellström C, Mattsson C, Schwenk JM, Nilsson P, Ayoglu B

Methods Mol. Biol. 1785 (-) 231-238 [2018-05-02; online 2018-05-02]

With the increasing availability of collections of antibodies, their evaluation in terms of binding selectivity becomes an important but challenging task. Planar antigen microarrays are very suitable tools to address this task and provide a powerful proteomics platform for the characterization of the binding selectivity of antibodies toward thousands of antigens in parallel. In this chapter, we describe our in-house developed procedures for the generation of high-density planar antigen microarrays with over 21,000 features. We also provide the details of the assay protocol, which we routinely use for the assessment of binding selectivity of the polyclonal antibodies generated within the Human Protein Atlas.

Affinity Proteomics Stockholm [Technology development]

Autoimmunity and Serology Profiling [Technology development]

Bioinformatics Support for Computational Resources [Service]

PubMed 29714022

DOI 10.1007/978-1-4939-7841-0_15

Crossref 10.1007/978-1-4939-7841-0_15