Targeted Long-Read Sequencing of a Locus Under Long-Term Balancing Selection in Capsella

Bachmann JA, Tedder A, Laenen B, Steige KA, Slotte T

G3 (Bethesda) 8 (4) 1327-1333 [2018-02-23; online 2018-02-23]

Rapid advances in short-read DNA sequencing technologies have revolutionized population genomic studies, but there are genomic regions where this technology reaches its limits. Limitations mostly arise due to the difficulties in assembly or alignment to genomic regions of high sequence divergence and high repeat content, which are typical characteristics for loci under strong long-term balancing selection. Studying genetic diversity at such loci therefore remains challenging. Here, we investigate the feasibility and error rates associated with targeted long-read sequencing of a locus under balancing selection. For this purpose, we generated bacterial artificial chromosomes (BACs) containing the Brassicaceae

NGI Uppsala (SNP&SEQ Technology Platform) [Service]

NGI Uppsala (Uppsala Genome Center) [Service]

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PubMed 29476024

DOI 10.1534/g3.117.300467

Crossref 10.1534/g3.117.300467

BioProject PRJEB24927 [Targeted long-read sequencing of a locus under long-term balancing selection in Capsella]