JSON
Soares RRG, García-Soriano DA, Larsson J, Fange D, Schirman D, Grillo M, Knöppel A, Sen BC, Svahn F, Zikrin S, Ratz M, Nilsson M, Elf J
Commun Biol 8 (1) 851 [2025-06-03; online 2025-06-03]
Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli. The method is applied to measure the intracellular maturation time of 84 red fluorescent proteins.
Bioinformatics Support for Computational Resources [Service]
PubMed 40461651
DOI 10.1038/s42003-025-08268-5
Crossref 10.1038/s42003-025-08268-5
pmc: PMC12134211
pii: 10.1038/s42003-025-08268-5