Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development.

Vain T, Raggi S, Ferro N, Barange DK, Kieffer M, Ma Q, Doyle SM, Thelander M, Pařízková B, Novák O, Ismail A, Enquist PA, Rigal A, Łangowska M, Ramans Harborough S, Zhang Y, Ljung K, Callis J, Almqvist F, Kepinski S, Estelle M, Pauwels L, Robert S

Proc. Natl. Acad. Sci. U.S.A. 116 (13) 6463-6472 [2019-03-26; online 2019-03-08]

Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCF TIR1/AFB functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Here, we report a chemical biology screen that identifies small molecules requiring AXR1 to modulate plant development. We selected four molecules of interest, RubNeddin 1 to 4 (RN1 to -4), among which RN3 and RN4 trigger selective auxin responses at transcriptional, biochemical, and morphological levels. This selective activity is explained by their ability to consistently promote the interaction between TIR1 and a specific subset of AUX/IAA proteins, stimulating the degradation of particular AUX/IAA combinations. Finally, we performed a genetic screen using RN4, the RN with the greatest potential for dissecting auxin perception, which revealed that the chromatin remodeling ATPase BRAHMA is implicated in auxin-mediated apical hook development. These results demonstrate the power of selective auxin agonists to dissect auxin perception for plant developmental functions, as well as offering opportunities to discover new molecular players involved in auxin responses.

Chemical Biology Consortium Sweden (CBCS) [Collaborative]

Swedish Metabolomics Centre (SMC) [Service]

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PubMed 30850516

DOI 10.1073/pnas.1809037116

Crossref 10.1073/pnas.1809037116

pii: 1809037116
pmc: PMC6442611