Sensitive detection of aggregated prion protein via proximity ligation.

Hammond M, Wik L, Deslys JP, Comoy E, Linné T, Landegren U, Kamali-Moghaddam M

Prion 8 (3) 261-265 [2014-12-09; online 2014-12-09]

The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of 3 or more copies of the specific protein. We have developed an SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at 100-fold higher concentration. Using either of the 2 monoclonal anti-PrP antibodies, 3F4 and 6H4, we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.

Affinity Proteomics Uppsala [Technology development]

Clinical Biomarkers

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PubMed 25482604

DOI 10.4161/pri.32231

Crossref 10.4161/pri.32231

pmc: PMC4601298