Systems-Level Immune Monitoring by Mass Cytometry.

Lakshmikanth T, Brodin P

Methods Mol. Biol. 1913 (-) 33-48 [2019-01-23; online 2019-01-23]

As therapies involving the modulation, stimulation, and deliberate excitation of the immune system are becoming routine, better methods for monitoring immune responses in human patients are needed. Mass cytometry allows for detailed profiling of all immune cell populations and their functional responses using a simple blood sample. When combined with appropriate computational analyses, the resolution for distinguishing desired responses from unproductive or even adverse reactions to immunotherapeutic interventions increases. Here we describe a core experimental and computational framework for global, systems-level immune monitoring by mass cytometry.

Mass Cytometry [Technology development]

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PubMed 30666597

DOI 10.1007/978-1-4939-8979-9_3

Crossref 10.1007/978-1-4939-8979-9_3