Allergen-specific human IgE isolated through an allergen-agnostic pipeline-understanding immune response and allergen recognition.
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Thörnqvist L, Franciskovic E, Godzwon M, Kristensen B, Sultan K, Nordström F, Palmason R, Todorovic N, Keller W, Lindstedt M, Greiff L, Levander F, Ohlin M
Commun Biol - (-) - [2026-01-28; online 2026-01-28]
Allergy, characterised by antibody responses of the IgE isotype, is a major health concern. The set of monoclonal human IgE used for studying the molecular mechanisms of allergies is limited. Single-cell sequencing offers opportunities to establish novel antibodies for researching, diagnosis, and treatment of allergies. We describe and exploit a pipeline for generating recombinant IgE directly from the immune repertoires of allergic subjects. It uses single-cell sequencing of IgM- B cells of bone marrow and peripheral blood in an allergen-agnostic manner, combined with high-throughput transcriptome sequencing to identify clonotypes populating the IgE repertoire. Immunochemical and immunoprecipitation analyses are used to deconvolute the specificity of identified antibodies. High-affinity antibodies were raised against four grass pollen allergens, antibodies that illustrated aspects of the development of allergen-specific humoral immunity. The pipeline provides a streamlined approach for the development and characterisation of native allergen-specific antibodies as they occur in allergy and during allergy desensitisation.
NGI Stockholm (Genomics Production) [Service]
National Genomics Infrastructure [Service]
PubMed 41606257
DOI 10.1038/s42003-026-09600-3
Crossref 10.1038/s42003-026-09600-3
pii: 10.1038/s42003-026-09600-3