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Dahmane S, Schexnaydre E, Zhang J, Singh BK, Rosendal E, Chotiwan N, Sharma KB, Nilsson E, Peters MBA, Yau WL, Rönfeldt S, Lundmark R, Barad BA, Grotjahn DA, Liese S, Carlson A, Överby AK, Carlson LA
Nat Commun 17 (1) 828 [2026-01-20; online 2026-01-20]
Flaviviruses replicate their genomes in replication organelles (ROs) formed as bud-like invaginations on the endoplasmic reticulum membrane, which also functions as the site for virion assembly. While this localization is well established, it is not known to what extent viral membrane remodeling, genome replication, virion assembly, and maturation are coordinated. Here, we image tick-borne flavivirus replication in human cells using cryo-electron tomography. We find that the RO membrane bud is shaped by a combination of a curvature-establishing membrane modification and the pressure from intraluminal template RNA. A protein complex at the RO base extends to an adjacent membrane, where immature virus particles bud. Naturally occurring furin site variants determine whether virus particles mature in the immediate vicinity of ROs. We further visualize replication in mouse brain tissue by cryo-electron tomography. Taken together, these findings reveal a close spatial coupling of flavivirus genome replication, budding, and maturation.
PubMed 41559045
DOI 10.1038/s41467-026-68483-4
Crossref 10.1038/s41467-026-68483-4
pmc: PMC12824359
pii: 10.1038/s41467-026-68483-4