{"entity": "publication", "iuid": "a9f5e65b684848218600bf30df2a82fc", "timestamp": "2026-08-12T05:35:29.850Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a9f5e65b684848218600bf30df2a82fc.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a9f5e65b684848218600bf30df2a82fc"}}, "title": "High-resolution model of Arabidopsis Photosystem II reveals the structural consequences of digitonin-extraction.", "authors": [{"family": "Gra\u00e7a", "given": "Andr\u00e9 T", "initials": "AT"}, {"family": "Hall", "given": "Michael", "initials": "M", "orcid": "0000-0003-0864-9798", "researcher": {"href": "https://publications.scilifelab.se/researcher/7489a92a241f4d0d9f8ef6eb4fcb3858.json"}}, {"family": "Persson", "given": "Karina", "initials": "K", "orcid": "0000-0003-0807-0348", "researcher": {"href": "https://publications.scilifelab.se/researcher/75b999cf004141468eefb2504b7c5b99.json"}}, {"family": "Schr\u00f6der", "given": "Wolfgang P", "initials": "WP"}], "type": "journal article", "published": "2021-07-30", "journal": {"title": "Sci Rep", "issn": "2045-2322", "issn-l": "2045-2322", "volume": "11", "issue": "1", "pages": "15534"}, "abstract": "In higher plants, the photosynthetic process is performed and regulated by Photosystem II (PSII). Arabidopsis thaliana was the first higher plant with a fully sequenced genome, conferring it the status of a model organism; nonetheless, a high-resolution structure of its Photosystem II is missing. We present the first Cryo-EM high-resolution structure of Arabidopsis PSII supercomplex with average resolution of 2.79 \u00c5, an important model for future PSII studies. The digitonin extracted PSII complexes demonstrate the importance of: the LHG2630-lipid-headgroup in the trimerization of the light-harvesting complex II; the stabilization of the PsbJ subunit and the CP43-loop E by DGD520-lipid; the choice of detergent for the integrity of membrane protein complexes. Furthermore, our data shows at the anticipated Mn4CaO5-site a single metal ion density as a reminiscent early stage of Photosystem II photoactivation.", "doi": "10.1038/s41598-021-94914-x", "pmid": "34330992", "labels": {"Cryo-EM": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41598-021-94914-x"}, {"db": "pmc", "key": "PMC8324835"}], "notes": [], "created": "2021-08-31T09:10:56.616Z", "modified": "2023-12-04T10:17:35.530Z"}