Genome-wide identification of modulators of Chlamydia trachomatis parasitophorous vacuole stability highlights an important role for sphingolipid supply.

Babu Sait MR, Jachmann LH, Türköz G, Milivojevic M, Llorente-Sáez C, Dhanjal S, Schumacher F, Henriksson S, Gayathri Vegesna NV, Seddik N, Chaban A, Mohanty P, Ölander M, Muraleedharan S, Farmand Azadeh S, Kleuser B, Schmierer B, Sixt BS

PLoS Biol. 23 (8) e3003297 [2025-08-00; online 2025-08-12]

A mechanistic understanding of how intracellular pathogens evade the intrinsic defenses of their host cells could open up intriguing therapeutic opportunities. Here, we applied a genome-wide genetic screening approach to investigate the nature of the defensive host cell death response suppressed by the membrane trafficking modulator CpoS, an effector protein secreted by the obligate intracellular bacterial pathogen Chlamydia trachomatis. Initially, this work revealed a CpoS-deficient mutant to exhibit a markedly increased dependence on host cellular synthesis of ceramides, the precursors of complex sphingolipids. Using novel microscopic reporters, we then established CpoS' role in defense evasion to occur by preserving the integrity of Chlamydia's parasitophorous vacuole (the inclusion) via ensuring an adequate sphingolipid supply. More specifically, we observed CpoS deficiency to destabilize inclusions, initially characterized by a release of individual bacteria into the host cell cytosol, then followed by inclusion rupture concomitant with host cell death. Exogenous addition of sphingosine stabilized CpoS-deficient inclusions, whereas disruption of host cellular ceramide synthesis destabilized wild-type inclusions. In combination, CpoS deficiency and impaired ceramide synthesis - presumably disrupting both Chlamydia's vesicular and non-vesicular sphingolipid supply routes - destabilized inclusions even earlier, resulting in infection clearance and host cell survival rather than host cell death. Overall, this study highlights how the vacuolar pathogen C. trachomatis maintains vacuole integrity by ensuring a steady sphingolipid supply, potentially offering inspiration and directions for future therapeutic strategies targeting parasitophorous vacuoles.

CRISPR Functional Genomics [Service]

Chemical Biology Consortium Sweden (CBCS) [Service]

Integrated Microscopy Technologies Umeå [Technology development]

PubMed 40794560

DOI 10.1371/journal.pbio.3003297

Crossref 10.1371/journal.pbio.3003297

pmc: PMC12342332
pii: PBIOLOGY-D-24-02720