#dc-system-status for updates. The development team can be contacted at datacentre@scilifelab.se if you have any question.Chromosome-level genome assembly of the morabine grasshopper Vandiemenella viatica19.
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Li X, Jayaprasad S, Einarsdottir E, Cooper SJB, Suh A, Kawakami T, Palacios-Gimenez OM
Sci Data 11 (1) 997 [2024-09-12; online 2024-09-12]
Morabine grasshoppers in the Vandiemenella viatica species group, which show karyotype diversity, have been studied for their ecological distribution and speciation in relation to their genetic and chromosomal diversity. They are good models for studying sex chromosome evolution as "old" and newly emerged sex chromosomes co-exist within the group. Here we present a reference genome for the viatica19 chromosomal race, that possesses the ancestral karyotype within the group. Using PacBio HiFi and Hi-C sequencing, we generated a chromosome-level assembly of 4.09 Gb in span, scaffold N50 of 429 Mb, and complete BUSCO score of 98.1%, containing 10 pseudo-chromosomes. We provide Illumina datasets of males and females, used to identify the X chromosome. The assembly contains 19,034 predicted protein-coding genes, and a total of 75.21% of repetitive DNA sequences. By leveraging HiFi reads, we mapped the genome-wide distribution of methylated bases (5mC and 6 mA). This comprehensive assembly offers a robust reference for morabine grasshoppers and supports further research into speciation and sex chromosome diversification within the group and its related species.
NGI Stockholm (Genomics Production) [Collaborative]
NGI Uppsala (Uppsala Genome Center) [Service]
National Genomics Infrastructure [Service]
PubMed 39266578
DOI 10.1038/s41597-024-03858-0
Crossref 10.1038/s41597-024-03858-0
pmc: PMC11393057
pii: 10.1038/s41597-024-03858-0