A multi-step peptidolytic cascade for amino acid recovery in chloroplasts.

Teixeira PF, Kmiec B, Branca RM, Murcha MW, Byzia A, Ivanova A, Whelan J, Drag M, Lehtiƶ J, Glaser E

Nat. Chem. Biol. 13 (1) 15-17 [2017-01-00; online 2016-10-31]

Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.

Clinical Proteomics Mass spectrometry [Collaborative]

Global Proteomics and Proteogenomics [Collaborative]

Swedish Metabolomics Centre (SMC) [Service]

PubMed 27820795

DOI 10.1038/nchembio.2227

Crossref 10.1038/nchembio.2227

pii: nchembio.2227

Publications 9.5.0