Solid-phase capture and profiling of open chromatin by spatial ATAC.

JSON

Llorens-Bobadilla E, Zamboni M, Marklund M, Bhalla N, Chen X, Hartman J, Frisén J, Ståhl PL

Nat. Biotechnol. 41 (8) 1085-1088 [2023-08-00; online 2023-01-05]

Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.

Bioinformatics Support for Computational Resources [Service]

NGI Short read [Service]

NGI Spatial omics

NGI Stockholm (Genomics Production) [Service]

National Genomics Infrastructure [Service]

PubMed 36604544

DOI 10.1038/s41587-022-01603-9

Crossref 10.1038/s41587-022-01603-9

pmc: PMC10421738
pii: 10.1038/s41587-022-01603-9