Multiplex protein detection with DNA readout via mass spectrometry.

Flanigon J, Kamali-Moghaddam M, Burbulis I, Annink C, Steffen M, Oeth P, Brent R, van den Boom D, Landegren U, Cantor C

N Biotechnol 30 (2) 153-158 [2013-01-25; online 2012-12-04]

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

PLA Proteomics

QC bibliography QC xrefs

PubMed 23201185

DOI 10.1016/j.nbt.2012.11.003

Crossref 10.1016/j.nbt.2012.11.003

mid NIHMS425130

S1871-6784(12)00860-6

pmc PMC3616745