One-Pot Time-Induced Proteome Integral Solubility Alteration Assay for Automated and Sensitive Drug-Target Identification.

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Meng Z, Saei AA, Lyu H, Gaetani M, Zubarev RA

Anal. Chem. - (-) - [2024-11-20; online 2024-11-20]

The proteome integral solubility alteration (PISA) assay is widely used for identifying drug targets, but it is labor-intensive and time-consuming and requires a substantial amount of biological sample. Aiming at enabling automation and greatly reducing the sample amount, we developed one-pot time-induced (OPTI)-PISA. Here, we demonstrate OPTI-PISA performance on identifying targets of multiple drugs in cell lysate and scaling down the sample amount to sub-microgram levels, making the PISA method suitable for NanoProteomics. OPTI-PISA can be implemented using only the standard equipment of a proteomics lab.

Chemical Proteomics [Technology development]

PubMed 39567183

DOI 10.1021/acs.analchem.4c05127

Crossref 10.1021/acs.analchem.4c05127