Effect of CTLA4-Ig (abatacept) Treatment on T cells and B cells in Peripheral Blood of Patients with Polymyositis and Dermatomyositis.

Tang Q, Ramsk├Âld D, Krystufkova O, Mann HF, Wick C, Dastmalchi M, Tadepally L, Yang C, Mikes J, Alexanderson H, Achour A, Brodin P, Vencovsky J, Lundberg IE, Malmstr├Âm V

Scand. J. Immunol. - (-) e12732 [2018-11-19; online 2018-11-19]

We aimed to evaluate in vivo effects of abatacept on phenotypes of T and B cells in the circulation of myositis patients in a sub-study of the ARTEMIS trial. Twelve patients with paired frozen PBMCs before and after 6-month abatacept treatment were included in this sub-study where mass cytometry (CyTOF) was chosen as a technology to be tested for its utility in a real life clinical immune-monitoring setting. Using CyTOF, the peripheral T cell phenotypes demonstrated considerable variation over time and between individuals precluding the identification of treatment-specific changes. We therefore conclude that studies of patient cohorts displaying wide clinical heterogeneity using mass cytometry must be relatively large in order to be suited for discovery research and immune-monitoring. Still, we did find some correlations with functional muscle outcome, namely positive correlations between the ratio of CD4

Bioinformatics Compute and Storage [Service]

Cellular Immunomonitoring [Service]

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PubMed 30451307

DOI 10.1111/sji.12732

Crossref 10.1111/sji.12732