Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics

Šimura J, Antoniadi I, Široká J, Tarkowská D, Strnad M, Ljung K, Novák O

Plant Physiol. 177 (2) 476-489 [2018-06-13; online 2018-04-27]

Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis ( Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.

Swedish Metabolomics Centre (SMC) [Service]

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PubMed 29703867

DOI 10.1104/pp.18.00293

Crossref 10.1104/pp.18.00293

pii: pp.18.00293
pmc: PMC6001343