A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection.

Yan J, Horák D, Lenfeld J, Hammond M, Kamali-Moghaddam M

J. Biotechnol. 167 (3) 235-240 [2013-09-10; online 2013-07-03]

Magnetic bead cellulose (MBC) was prepared using sol-gel transition of viscose in the presence of maghemite (γ-Fe₂O₃) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA.

Affinity Proteomics Uppsala [Technology development]

PLA and Single Cell Proteomics

PubMed 23811391

DOI 10.1016/j.jbiotec.2013.06.010

Crossref 10.1016/j.jbiotec.2013.06.010

pii: S0168-1656(13)00266-6

Publications 9.5.0