Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress.

Butler È, Alsterfjord M, Olofsson TC, Karlsson C, Malmström J, Vásquez A

BMC Microbiol. 13 (-) 235 [2013-10-22; online 2013-10-22]

Lactic acid bacteria (LAB) has been considered a beneficial bacterial group, found as part of the microbiota of diverse hosts, including humans and various animals. However, the mechanisms of how hosts and LAB interact are still poorly understood. Previous work demonstrates that 13 species of Lactobacillus and Bifidobacterium from the honey crop in bees function symbiotically with the honeybee. They protect each other, their hosts, and the surrounding environment against severe bee pathogens, bacteria, and yeasts. Therefore, we hypothesized that these LAB under stress, i.e. in their natural niche in the honey crop, are likely to produce bioactive substances with antimicrobial activity. The genomic analysis of the LAB demonstrated varying genome sizes ranging from 1.5 to 2.2 mega-base pairs (Mbps) which points out a clear difference within the protein gene content, as well as specialized functions in the honeybee microbiota and their adaptation to their host. We demonstrate a clear variation between the secreted proteins of the symbiotic LAB when subjected to microbial stressors. We have identified that 10 of the 13 LAB produced extra-cellular proteins of known or unknown function in which some are arranged in interesting putative operons that may be involved in antimicrobial action, host interaction, or biofilm formation. The most common known extra-cellular proteins secreted were enzymes, DNA chaperones, S-layer proteins, bacteriocins, and lysozymes. A new bacteriocin may have been identified in one of the LAB symbionts while many proteins with unknown functions were produced which must be investigated further. The 13 LAB symbionts likely play different roles in their natural environment defending their niche and their host and participating in the honeybee's food production. These roles are partly played through producing extracellular proteins on exposure to microbial stressors widely found in natural occurring flowers. Many of these secreted proteins may have a putative antimicrobial function. In the future, understanding these processes in this complicated environment may lead to novel applications of honey crop LAB proteins.

Bioinformatics Support and Infrastructure

QC bibliography QC xrefs

PubMed 24148670

DOI 10.1186/1471-2180-13-235

Crossref 10.1186/1471-2180-13-235

GENBANK KC776061

GENBANK KC776062

GENBANK KC776063

GENBANK KC776064

GENBANK KC776065

GENBANK KC776066

GENBANK KC776067

GENBANK KC776068

GENBANK KC776069

GENBANK KC776070

GENBANK KC776071

GENBANK KC776073

GENBANK KC776074

GENBANK KC776075

GENBANK KC776076

GENBANK KC776077

GENBANK KC776078

GENBANK KC776079

GENBANK KC776080

GENBANK KC776081

GENBANK KC776082

GENBANK KC776083

GENBANK KC776084

GENBANK KC776085

GENBANK KC776086

GENBANK KC776087

GENBANK KC776088

GENBANK KC776089

GENBANK KC776090

GENBANK KC776091

GENBANK KC776092

GENBANK KC776093

GENBANK KC776094

GENBANK KC776095

GENBANK KC776096

GENBANK KC776097

GENBANK KC776098

GENBANK KC776099

GENBANK KC776100

GENBANK KC776101

GENBANK KC776102

GENBANK KC776103

GENBANK KC776104

GENBANK KC776105

GENBANK KC776106

GENBANK KC776107

GENBANK KC776108

GENBANK KC776109

GENBANK KC776110

GENBANK KC776111

GENBANK KC789963

GENBANK KC789964

GENBANK KC789965

GENBANK KC789966

GENBANK KC789967

GENBANK KC789968

GENBANK KC789969

GENBANK KC789970

GENBANK KC789971

GENBANK KC789972

GENBANK KC789973

GENBANK KC789974

GENBANK KC789975

GENBANK KC789976

GENBANK KC789977

GENBANK KC789978

GENBANK KC789979

GENBANK KC789980

GENBANK KC789981

GENBANK KC789982

GENBANK KC789983

GENBANK KC789984

GENBANK KC789985

GENBANK KC789986

GENBANK KC789987

GENBANK KC789988

GENBANK KC789989

GENBANK KC789990

GENBANK KC789991

GENBANK KC789992

GENBANK KC789993

GENBANK KC789994

GENBANK KC789995

GENBANK KC789996

GENBANK KC789997

GENBANK KC789998

GENBANK KC789999

GENBANK KC790000

GENBANK KC790001

GENBANK KC790002

GENBANK KC790003

GENBANK KC790004

GENBANK KC790005

GENBANK KC790006

GENBANK KC790007

GENBANK KC790008

GENBANK KC790009

GENBANK KC790010

GENBANK KC790011

GENBANK KC790012

GENBANK KC790013

GENBANK KC790014

GENBANK KC790015

GENBANK KC790016

GENBANK KC790017

GENBANK KC790018

GENBANK KC801035

1471-2180-13-235

pmc PMC4015849