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Corvaisier M, Zhou J, Malycheva D, Cornella N, Chioureas D, Gustafsson NMS, Rosselló CA, Ayora S, Li T, Ekström-Holka K, Jirström K, Lindström L, Alvarado-Kristensson M
Commun Biol 4 (1) 767 [2021-06-22; online 2021-06-22]
Changes in the location of γ-tubulin ensure cell survival and preserve genome integrity. We investigated whether the nuclear accumulation of γ-tubulin facilitates the transport of proliferating cell nuclear antigen (PCNA) between the cytosolic and the nuclear compartment in mammalian cells. We found that the γ-tubulin meshwork assists in the recruitment of PCNA to chromatin. Also, decreased levels of γ-tubulin reduce the nuclear pool of PCNA. In addition, the γ-tubulin C terminus encodes a PCNA-interacting peptide (PIP) motif, and a γ-tubulin-PIP-mutant affects the nuclear accumulation of PCNA. In a cell-free system, PCNA and γ-tubulin formed a complex. In tumors, there is a significant positive correlation between TUBG1 and PCNA expression. Thus, we report a novel mechanism that constitutes the basis for tumor growth by which the γ-tubulin meshwork maintains indefinite proliferation by acting as an opportune scaffold for the transport of PCNA from the cytosol to the chromatin.
NGI Uppsala (SNP&SEQ Technology Platform) [Service]
National Genomics Infrastructure [Service]
PubMed 34158617
DOI 10.1038/s42003-021-02280-1
Crossref 10.1038/s42003-021-02280-1
pii: 10.1038/s42003-021-02280-1
pmc: PMC8219688