The transcriptome of the adenovirus infected cell.

Zhao H, Dahlö M, Isaksson A, Syvänen AC, Pettersson U

Virology 424 (2) 115-128 [2012-03-15; online 2012-01-13]

Alternations of cellular gene expression following an adenovirus type 2 infection of human primary cells were studied by using superior sensitive cDNA sequencing. In total, 3791 cellular genes were identified as differentially expressed more than 2-fold. Genes involved in DNA replication, RNA transcription and cell cycle regulation were very abundant among the up-regulated genes. On the other hand, genes involved in various signaling pathways including TGF-β, Rho, G-protein, Map kinase, STAT and NF-κB stood out among the down-regulated genes. Binding sites for E2F, ATF/CREB and AP2 were prevalent in the up-regulated genes, whereas binding sites for SRF and NF-κB were dominant among the down-regulated genes. It is evident that the adenovirus has gained a control of the host cell cycle, growth, immune response and apoptosis at 24 h after infection. However, efforts from host cell to block the cell cycle progression and activate an antiviral response were also observed.

Array and Analysis Facility

NGI Uppsala (SNP&SEQ Technology Platform)

National Genomics Infrastructure

PubMed 22236370

DOI 10.1016/j.virol.2011.12.006

Crossref 10.1016/j.virol.2011.12.006

pii: S0042-6822(11)00564-2

Publications 9.5.0