Hydrazide-based reactive matrices for the sensitive detection of aldehydes and ketones by MALDI mass spectrometry imaging.

Lodén H, Schembri LS, Nilsson A, Kaya I, Shariatgorji R, Odell LR, Andrén PE

Chem. Commun. (Camb.) 60 (69) 9238-9241 [2024-08-22; online 2024-08-22]

A one-step, on-tissue chemical derivatisation method for MALDI mass spectrometry imaging was found to improve the detectability of aldehydes and ketones by charge-tagging. The developed reactive matrices, containing a UV-chromophore, ionisable moiety and hydrazide group, showed an equal or higher detection efficiency than Girard's reagent P, enabling improved imaging of brain metabolites without the need for additional co-matrices.

Spatial Mass Spectrometry [Technology development]

PubMed 39114958

DOI 10.1039/d4cc02475c

Crossref 10.1039/d4cc02475c