A chromosome-level assembly of the seed beetle Callosobruchus maculatus genome with annotation of its repetitive elements.

Arnqvist G, Westerberg I, Galbraith J, Sayadi A, Scofield DG, Olsen RA, Immonen E, Bonath F, Ewels P, Suh A

G3 (Bethesda) - (-) - [2023-12-13; online 2023-12-13]

Callosobruchus maculatus is a major agricultural pest of legume crops worldwide and an established model system in ecology and evolution. Yet, current molecular biological resources for this species are limited. Here, we employ Hi-C sequencing to generate a greatly improved genome assembly and we annotate its repetitive elements in a dedicated in-depth effort where we manually curate and classify the most abundant unclassified repeat subfamilies. We present a scaffolded chromosome-level assembly, which is 1.01 Gb in total length with 86% being contained within the 9 autosomes and the X chromosome. Repetitive sequences accounted for 70% of the total assembly. DNA transposons covered 18% of the genome, with the most abundant superfamily being Tc1-Mariner (9.75% of the genome). This new chromosome-level genome assembly of C. maculatus will enable future genetic and evolutionary studies not only of this important species but of beetles more generally.

Bioinformatics Support for Computational Resources [Service]

NGI Other [Service]

NGI Short read [Service]

NGI Stockholm (Genomics Applications) [Collaborative]

NGI Stockholm (Genomics Production) [Service]

National Genomics Infrastructure [Service]

PubMed 38092066

DOI 10.1093/g3journal/jkad266

Crossref 10.1093/g3journal/jkad266

pii: 7471854

Publications 9.5.0