Single-cell RNA sequencing of mouse brain and lung vascular and vessel-associated cell types.

He L, Vanlandewijck M, Mäe MA, Andrae J, Ando K, Del Gaudio F, Nahar K, Lebouvier T, Laviña B, Gouveia L, Sun Y, Raschperger E, Segerstolpe Å, Liu J, Gustafsson S, Räsänen M, Zarb Y, Mochizuki N, Keller A, Lendahl U, Betsholtz C

Sci Data 5 (-) 180160 [2018-08-21; online 2018-08-21]

Vascular diseases are major causes of death, yet our understanding of the cellular constituents of blood vessels, including how differences in their gene expression profiles create diversity in vascular structure and function, is limited. In this paper, we describe a single-cell RNA sequencing (scRNA-seq) dataset that defines vascular and vessel-associated cell types and subtypes in mouse brain and lung. The dataset contains 3,436 single cell transcriptomes from mouse brain, which formed 15 distinct clusters corresponding to cell (sub)types, and another 1,504 single cell transcriptomes from mouse lung, which formed 17 cell clusters. In order to allow user-friendly access to our data, we constructed a searchable database (http://betsholtzlab.org/VascularSingleCells/database.html). Our dataset constitutes a comprehensive molecular atlas of vascular and vessel-associated cell types in the mouse brain and lung, and as such provides a strong foundation for future studies of vascular development and diseases.

Bioinformatics Compute and Storage [Service]

Eukaryotic Single Cell Genomics (ESCG) [Service]

NGI Stockholm (Genomics Applications) [Service]

NGI Stockholm (Genomics Production) [Service]

QC bibliography QC xrefs

PubMed 30129931

DOI 10.1038/sdata.2018.160

Crossref 10.1038/sdata.2018.160

sdata2018160

pmc PMC6103262

GEO GSE99058 [Single cell RNA-seq of mouse brain astrocyte transcriptomes]

GEO GSE98816 [Single cell RNA-seq of mouse brain vascular transcriptomes]

GEO GSE99235 [Single cell RNA-seq of mouse lung vascular transcriptomes]

https://doi.org/10.6084/m9.figshare.c.4077260). [Single cell RNAseq of mouse brain and lung vascular and vessel-associated cell types]