Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing.

Brofelth M, Ekstrand AI, Gour S, Jansson R, Hedhammar M, Elleby B, Kvist A, Wingren C, Axelsson U, Borrebaeck CAK

Commun Biol 3 (1) 339 [2020-07-03; online 2020-07-03]

The composition of serum proteins is reflecting the current health status and can, with the right tools, be used to detect early signs of disease, such as an emerging cancer. An earlier diagnosis of cancer would greatly increase the chance of an improved outcome for the patients. However, there is still an unmet need for proficient tools to decipher the information in the blood proteome, which calls for further technological development. Here, we present a proof-of-concept study that demonstrates an alternative approach for multiplexed protein profiling of serum samples in solution, using DNA barcoded scFv antibody fragments and next generation sequencing. The outcome shows high accuracy when discriminating samples derived from pancreatic cancer patients and healthy controls and represents a scalable alternative for serum analysis.

Clinical Genomics Lund

PubMed 32620783

DOI 10.1038/s42003-020-1068-0

Crossref 10.1038/s42003-020-1068-0

pii: 10.1038/s42003-020-1068-0
pmc: PMC7334203
figshare: 10.6084/m9.figshare.12370106

Publications 9.5.0