Wiley Analytical Science https://analyticalscience.wiley.com/do/10.1002/was.000400044 (-) - [2020-11-30; online 2020-05-26]
Time-lapse imaging allows researchers to follow various processes over time. Applications in life sciences can be as varied as evaluating the role of cell division and migration in developing embryos to the measurement of the moving pattern of living mice. In both of these example cases the process can be quantified using single particle tracking. A very powerful but still easy to use tool for single particle tracking is TrackMate – which comes as a plugin within Fiji [1,2]. This article gives an overview of the functionalities of TrackMate.