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Sato K, Lyu J, van den Berg J, Braat D, Cruz VM, Navarro Luzón C, Schimmel J, Esteban-Jurado C, Alemany M, Dreyer J, Hendrikx A, Mattiroli F, van Oudenaarden A, Tijsterman M, Elsässer SJ, Knipscheer P
Science 388 (6752) 1225-1231 [2025-06-12; online 2025-06-12]
G-quadruplexes (G4s) are prevalent DNA structures that regulate transcription but also threaten genome stability. How G4 dynamics are controlled remains poorly understood. Here, we report that RNA transcripts govern G4 landscapes through coordinated G-loop assembly and disassembly. G-loop assembly involves activation of the ATM and ATR kinases, followed by homology-directed invasion of RNA opposite the G4 strand mediated by BRCA2 and RAD51. Disassembly of the G-loop resolves the G4 structure through DHX36-FANCJ-mediated G4 unwinding, which triggers nucleolytic incision and subsequent hybrid strand renewal by DNA synthesis. Inhibition of G-loop disassembly causes global G4 and R-loop accumulation, leading to transcriptome dysregulation, replication stress, and genome instability. These findings establish an intricate G-loop assembly-disassembly mechanism that controls G4 landscapes and is essential for cellular homeostasis and survival.
Bioinformatics Support for Computational Resources [Service]
PubMed 40504899
DOI 10.1126/science.adr0493
Crossref 10.1126/science.adr0493