T-RHEX-RNAseq - a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells.

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Gustafsson C, Hauenstein J, Frengen N, Krstic A, Luc S, Månsson R

BMC Genomics 24 (1) 205 [2023-04-17; online 2023-04-17]

RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming - to allow analysis of a broader range of transcripts - remains challenging. We here describe a tagmentation-based, rRNA blocked, random hexamer primed RNAseq approach (T-RHEX-RNAseq) for generating stranded RNAseq libraries from very low numbers of FACS sorted cells without RNA purification steps. T-RHEX-RNAseq provides an easy-to-use, time efficient and automation compatible method for generating stranded RNAseq libraries from rare cells.

Bioinformatics Support for Computational Resources [Service]

PubMed 37069502

DOI 10.1186/s12864-023-09279-4

Crossref 10.1186/s12864-023-09279-4

pmc: PMC10111750
pii: 10.1186/s12864-023-09279-4