Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response.

Vanherberghen B, Frisk T, Forslund E, Olofsson PE, Guldevall K, Önfelt B

Methods Mol. Biol. 1441 (-) 87-106 [2016-05-15; online 2016-05-15]

NK cell heterogeneity has primarily been studied either on the population level, measuring average responses, or on the single cell level by flow cytometry, providing static snapshots. These approaches have certain drawbacks, not enabling dynamic observations of single cells over extended periods of time. One of the primary limitations of single cell imaging has been throughput; it has been challenging to collect data for many cells due to their dynamic nature and migrating out of the field of view. Spatially confining cells combined with automated fluorescence microscopy enables the simultaneous monitoring of many NK cells in parallel for extended periods of time (>12 h). Such an approach allows us to dissect how the sum of individual NK cell responses translates to the global average response typically observed.

Advanced Light Microscopy (ALM) [Technology development]

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PubMed 27177659

DOI 10.1007/978-1-4939-3684-7_8

Crossref 10.1007/978-1-4939-3684-7_8