Chemical fragmentation for massively parallel sequencing library preparation.

Gyarmati P, Song Y, Hällman J, Käller M

J. Biotechnol. 168 (1) 95-100 [2013-10-10; online 2013-09-03]

Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron-EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation.

NGI Stockholm (Genomics Applications)

NGI Stockholm (Genomics Production)

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PubMed 23994687

DOI 10.1016/j.jbiotec.2013.08.020

Crossref 10.1016/j.jbiotec.2013.08.020

S0168-1656(13)00365-9