Chemical fragmentation for massively parallel sequencing library preparation.

Gyarmati P, Song Y, Hällman J, Käller M

J. Biotechnol. 168 (1) 95-100 [2013-10-10; online 2013-09-03]

Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron-EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation.

NGI Stockholm (Genomics Applications)

NGI Stockholm (Genomics Production)

National Genomics Infrastructure

PubMed 23994687

DOI 10.1016/j.jbiotec.2013.08.020

Crossref 10.1016/j.jbiotec.2013.08.020

pii: S0168-1656(13)00365-9

Publications 9.5.0