{"entity": "publication", "iuid": "c522f5cf12954e2ca97ffac12e5e0bfb", "timestamp": "2026-08-12T05:50:49.132Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c522f5cf12954e2ca97ffac12e5e0bfb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c522f5cf12954e2ca97ffac12e5e0bfb"}}, "title": "Chemical fragmentation for massively parallel sequencing library preparation.", "authors": [{"family": "Gyarmati", "given": "P", "initials": "P"}, {"family": "Song", "given": "Y", "initials": "Y"}, {"family": "H\u00e4llman", "given": "J", "initials": "J"}, {"family": "K\u00e4ller", "given": "M", "initials": "M", "orcid": "0000-0001-6813-3051", "researcher": {"href": "https://publications.scilifelab.se/researcher/536ad902a272482aba853c078557e240.json"}}], "type": "journal article", "published": "2013-10-10", "journal": {"volume": "168", "issn": "1873-4863", "issue": "1", "pages": "95-100", "title": "J. Biotechnol.", "issn-l": "0168-1656"}, "abstract": "Fragmentation is essential in most library preparation protocols for use with massively parallel sequencing systems. Complexes that generate hydroxyl radicals, such as iron-EDTA, can be used to introduce random DNA cleavage. Here we describe a chemical fragmentation method that can be incorporated into library preparation protocols for next-generation sequencing workflows. This protocol has been validated by whole genome, amplicon and exome sequencing. Chemical fragmentation is a cost-effective alternative to current fragmentation methods that has no observable sequence bias and requires no instrumentation.", "doi": "10.1016/j.jbiotec.2013.08.020", "pmid": "23994687", "labels": {"National Genomics Infrastructure": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null}, "xrefs": [{"db": "pii", "key": "S0168-1656(13)00365-9"}], "notes": [], "created": "2017-05-04T14:57:59.697Z", "modified": "2021-07-07T15:22:42.796Z"}