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MacLean AE, Shikha S, Ferreira Silva M, Gramelspacher MJ, Nilsen A, Liebman KM, Pou S, Winter RW, Meir A, Riscoe MK, Doggett JS, Sheiner L, Mühleip A
Nat. Struct. Mol. Biol. 32 (8) 1424-1433 [2025-08-00; online 2025-05-19]
The apicomplexan mitochondrial electron transport chain is essential for parasite survival and displays a divergent subunit composition. Here we report cryo-electron microscopy structures of an apicomplexan III2-IV supercomplex and of the drug target complex III2. The supercomplex structure reveals how clade-specific subunits form an apicomplexan-conserved III2-IV interface with a unique, kinked architecture, suggesting that supercomplexes evolved independently in different eukaryotic lineages. A knockout resulting in supercomplex disassembly challenges the proposed role of III2-IV in electron transfer efficiency as suggested for mammals. Nevertheless, knockout analysis indicates that III2-IV is critical for parasite fitness. The complexes from the model parasite Toxoplasma gondii were inhibited with the antimalarial atovaquone, revealing interactions underpinning species specificity. They were also inhibited with endochin-like quinolone (ELQ)-300, an inhibitor in late-stage preclinical development. Notably, in the apicomplexan binding site, ELQ-300 is flipped compared with related compounds in the mammalian enzyme. On the basis of the binding modes and parasite-specific interactions discovered, we designed more potent ELQs with subnanomolar activity against T. gondii. Our findings reveal critical evolutionary differences in the role of supercomplexes in mitochondrial biology and provide insight into cytochrome b inhibition, informing future drug discovery.
PubMed 40389671
DOI 10.1038/s41594-025-01531-7
Crossref 10.1038/s41594-025-01531-7
pmc: PMC12350165
pii: 10.1038/s41594-025-01531-7