JSON
Plant Reprod 35 (2) 153-160 [2022-06-00; online 2021-11-23]
Meiosis is a specialized cell division that is key for reproduction and genetic diversity in sexually reproducing plants. Recently, different RNA silencing pathways have been proposed to carry a specific activity during meiosis, but the pathways involved during this process remain unclear. Here, we explored the subcellular localization of different ARGONAUTE (AGO) proteins, the main effectors of RNA silencing, during male meiosis in Arabidopsis thaliana using immunolocalizations with commercially available antibodies. We detected the presence of AGO proteins associated with posttranscriptional gene silencing (AGO1, 2, and 5) in the cytoplasm and the nucleus, while AGOs associated with transcriptional gene silencing (AGO4 and 9) localized exclusively in the nucleus. These results indicate that the localization of different AGOs correlates with their predicted roles at the transcriptional and posttranscriptional levels and provide an overview of their timing and potential role during meiosis.
Bioinformatics Support for Computational Resources [Service]
PubMed 34812935
DOI 10.1007/s00497-021-00434-z
Crossref 10.1007/s00497-021-00434-z
pii: 10.1007/s00497-021-00434-z
pmc: PMC9110482