Single-cell RNA sequencing of cells from fresh or frozen tissue reveals a signature of freezing marked by heightened stress and activation.

Stamper CT, Marchalot A, Tibbitt CA, Weigel W, Jangard M, Theorell J, Mjösberg J

Eur. J. Immunol. 54 (4) e2350660 [2024-04-00; online 2024-02-02]

Thawing of viably frozen human tissue T cells, ILCs, and NK cells and subsequent single-cell RNA sequencing reveals that recovery of cellular subclusters is variably impacted. While freeze-thawing does not alter the transcriptional profiles of cells, it upregulates genes and gene pathways associated with stress and activation.

Bioinformatics Support for Computational Resources [Service]

PubMed 38304946

DOI 10.1002/eji.202350660

Crossref 10.1002/eji.202350660