A standardized protocol for comparable analysis of GSH/GSSG by UHPLC-ESI-MSMS for human plasma.

Claeson AS, Gouveia-Figueira S, Stenlund H, Johansson AI

Journal of Chromatography B 1104 (-) 67-72 [2019-01-01; online 2018-11-10]

Variability in the levels of GSH and GSSG in plasma is suggested to derive from inadequate pre-processing methods. The aim of this study was to develop a protocol for comparable and reliable measurements of GSH/GSSG. Venous blood from 8 healthy individuals were collected and divided into 7 different pre-processing procedures. For three of the samples an extraction mixture was added after 0 (baseline), 4 and 8 min and for three of the samples the extraction mixture was added at different times during defrost. A worst case scenario where a sample was left in a cool box during 6 h was also included. The samples were analyzed with UHPLC-ESI-MSMS. A large difference in the levels of GSH and GSSG were identified and it was clearly associated with the sample handling procedures. A sample left untreated for 4 min will have significantly reduced amount of GSH. Stability tests showed that the level of GSH was reduced after 3 months in -80 °C.

Swedish Metabolomics Centre (SMC) [Collaborative]

PubMed 30445289

DOI 10.1016/j.jchromb.2018.11.007

Crossref 10.1016/j.jchromb.2018.11.007

pii: S1570-0232(18)31199-1