Generation of Sequencing Libraries for Structural Analysis of Bacterial 5' UTRs.

Ignatov D, Vaitkevicius K, Johansson J

STAR Protoc 1 (2) 100046 [2020-09-18; online 2020-06-06]

The structure of 5' untranslated regions (5' UTRs) of bacterial mRNAs often determines the fate of the transcripts. Using a dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) approach, we developed a protocol to generate sequence libraries to determine the base-pairing status of adenines and cytosines in the 5' UTRs of bacterial mRNAs. Our method increases the sequencing depth of the 5' UTRs and allows detection of changes in their structures by sequencing libraries of moderate sizes. For complete details on the use and execution of this protocol, please refer to Ignatov et al. (2020).

Bioinformatics Compute and Storage [Service]

NGI Stockholm (Genomics Applications) [Service]

NGI Stockholm (Genomics Production) [Service]

National Genomics Infrastructure [Service]

PubMed 33111092

DOI 10.1016/j.xpro.2020.100046

Crossref 10.1016/j.xpro.2020.100046

pii: S2666-1667(20)30033-2
pmc: PMC7580233