Single-nucleus epigenomic profiling of the adult human central nervous system unveils epigenetic memory of developmental programs.
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Kabbe M, Agirre E, Carlström KE, Dumral Ö, Lor YK, Pohl FB, Ruffin N, van Bruggen D, Meijer M, Seeker LA, Bestard-Cuche N, Lederer AR, Zhang J, Ahola V, Goldman SA, Edström E, Arvidsson L, Moreira TH, Bartosovic M, Jagodic M, Williams A, Castelo-Branco G
Nat. Neurosci. - (-) - [2026-03-19; online 2026-03-19]
Neural cells in the adult human central nervous system (CNS) display extensive transcriptional heterogeneity. How different layers of epigenetic regulation underpin this heterogeneity is poorly understood. Here we profile, at the single-nuclei epigenomic level, distinct regions of the adult human CNS, for chromatin accessibility and simultaneously for the histone modifications H3K27me3 and H3K27ac. We unveil a putative SOX10 enhancer and primed chromatin signatures at HOX loci in spinal-cord-derived human oligodendroglia (OLG) and astrocytes, but not microglia. These signatures in adult OLG were reminiscent of developmental profiles but were decoupled from robust gene expression. Moreover, using high-resolution Micro-C, we show that induced pluripotent stem-cell-derived human OLGs exhibit a HOX chromatin architecture compatible with the primed chromatin in adult OLGs, bearing a strong resemblance not only to OLG developmental architecture but also to high-grade pontine gliomas. Thus, epigenetic memory from developmental states in adult OLG not only enables them to promptly transcribe Hox family genes during regeneration but also makes them susceptible to gliomagenesis.
NGI Stockholm (Genomics Production) [Service]
National Genomics Infrastructure [Service]
PubMed 41857393
DOI 10.1038/s41593-026-02208-0
Crossref 10.1038/s41593-026-02208-0
pii: 10.1038/s41593-026-02208-0