Read-based phasing of related individuals.

Garg S, Martin M, Marschall T

Bioinformatics 32 (12) i234-i242 [2016-06-15; online 2016-06-17]

Read-based phasing deduces the haplotypes of an individual from sequencing reads that cover multiple variants, while genetic phasing takes only genotypes as input and applies the rules of Mendelian inheritance to infer haplotypes within a pedigree of individuals. Combining both into an approach that uses these two independent sources of information-reads and pedigree-has the potential to deliver results better than each individually. We provide a theoretical framework combining read-based phasing with genetic haplotyping, and describe a fixed-parameter algorithm and its implementation for finding an optimal solution. We show that leveraging reads of related individuals jointly in this way yields more phased variants and at a higher accuracy than when phased separately, both in simulated and real data. Coverages as low as 2× for each member of a trio yield haplotypes that are as accurate as when analyzed separately at 15× coverage per individual.

Bioinformatics Long-term Support WABI [Technology development]

Bioinformatics Support, Infrastructure and Training [Technology development]

PubMed 27307622

DOI 10.1093/bioinformatics/btw276

Crossref 10.1093/bioinformatics/btw276

pii: btw276
pmc: PMC4908360

Publications 9.5.0