Live Cell FRET Imaging Reveals Amyloid β-Peptide Oligomerization in Hippocampal Neurons.

Gao Y, Wennmalm S, Winblad B, Schedin-Weiss S, Tjernberg LO

Int J Mol Sci 22 (9) 4530 [2021-04-26; online 2021-04-26]

Amyloid β-peptide (Aβ) oligomerization is believed to contribute to the neuronal dysfunction in Alzheimer disease (AD). Despite decades of research, many details of Aβ oligomerization in neurons still need to be revealed. Förster resonance energy transfer (FRET) is a simple but effective way to study molecular interactions. Here, we used a confocal microscope with a sensitive Airyscan detector for FRET detection. By live cell FRET imaging, we detected Aβ42 oligomerization in primary neurons. The neurons were incubated with fluorescently labeled Aβ42 in the cell culture medium for 24 h. Aβ42 were internalized and oligomerized in the lysosomes/late endosomes in a concentration-dependent manner. Both the cellular uptake and intracellular oligomerization of Aβ42 were significantly higher than for Aβ40. These findings provide a better understanding of Aβ42 oligomerization in neurons.

Integrated Microscopy Technologies Stockholm [Service]

PubMed 33926107

DOI 10.3390/ijms22094530

Crossref 10.3390/ijms22094530

pii: ijms22094530
pmc: PMC8123703

Publications 9.5.0