Short Chain Fatty Acids (SCFA) Reprogram Gene Expression in Human Malignant Epithelial and Lymphoid Cells.

Astakhova L, Ngara M, Babich O, Prosekov A, Asyakina L, Dyshlyuk L, Midtvedt T, Zhou X, Ernberg I, Matskova L

PLoS ONE 11 (7) e0154102 [2016-07-21; online 2016-07-21]

The effect of short chain fatty acids (SCFAs) on gene expression in human, malignant cell lines was investigated, with a focus on signaling pathways. The commensal microbial flora produce high levels of SCFAs with established physiologic effects in humans. The most abundant SCFA metabolite in the human microflora is n-butyric acid. It is well known to activate endogenous latent Epstein-Barr virus (EBV), that was used as a reference read out system and extended to EBV+ epithelial cancer cell lines. N-butyric acid and its salt induced inflammatory and apoptotic responses in tumor cells of epithelial and lymphoid origin. Epithelial cell migration was inhibited. The n-butyric gene activation was reduced by knock-down of the cell membrane transporters MCT-1 and -4 by siRNA. N-butyric acid show biologically significant effects on several important cellular functions, also with relevance for tumor cell phenotype.

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PubMed 27441625

DOI 10.1371/journal.pone.0154102

Crossref 10.1371/journal.pone.0154102

PONE-D-15-52288

pmc PMC4956219